经眶下神经注射ET-1 复制三叉神经痛大鼠模型

Study of trigeminal neuralgia in an animal model produced by administration of endothelin-1 to the infraorbital nerve

摘要:

目的 经眶下神经注射内皮素-1（ET-1）复制三叉神经痛大鼠模型。方法 实验1，将24 只 SD 大鼠随机分为A、B、C 3 组，A、B 组分别经眶下神经注射不同剂量的ET-1 溶液，C 组注射10μl 生理盐水， 观察各组大鼠给药后60 min 眶下神经支配区域机械痛阈值和抓脸行为的变化。实验2，将16 只SD 大鼠随机 分为两组，分别连续喂给卡马西平（D 组）、生理盐水（E 组）3 d，然后按实验1 的方法复制三叉神经痛模型， 观察注射ET-1 溶液后60 min 大鼠眶下神经支配区域机械痛阈值的变化。结果 实验1 中与C 组比较，A、 B 组术后60 min 大鼠眶下神经支配区域机械痛阈值降低，抓脸行为次数增多，且B 组变化更显著（P <0.05）。 实验2 中D 组大鼠术后对机械刺激的痛敏反应敏感性较E 组降低（P <0.05）。结论 经眶下神经注射ET-1 能复制理想的三叉神经痛大鼠模型。

Abstract:

Objective To establish a rat model of trigeminal neuralgia produced by administration of endothelin-1 (ET-1) to the infraorbital nerve. Methods Experiment 1: Twenty-four healthy Sprague-Dawley rats randomly divided into 3 groups: ET-1 8 nmol injection group (group A, n = 8), ET-1 16 nmol injection group (group B, n = 8), and saline 10μl injection group (group C, n = 8). The changes of mechanical pain threshold and the facial scratching behavior in infraorbital nerve innervation area had been observed during 60 minutes after surgery. Experiment 2: Sixteen healthy Sprague-Dawley rats randomly divided into carbamazepine fed group (group D, n = 8) and saline fed group (group E, n = 8). To establish model of trigeminal neuralgia according to the method of experiment 1 after continuous feeding for 3 days. The changes of mechanical pain threshold and the facial scratching behavior in infraorbital nerve innervation area had been observed during 60 minutes after surgery. Results Experiment 1: Compared with group C, the mechanical pain threshold of infraorbital nerve innervation area decreased and the number of facial scratching behavior increased in group A and B (P < 0.05), and the changes in group B were more significant (P < 0.05). Experiment 2: Compared with group E, the mechanical pain threshold of infraorbital nerve innervation area increased and the number of facial scratching behavior decreased in group D (P < 0.05). Conclusions Rat model of trigeminal neuralgia can be successfully established by injecting ET-1 into infraorbital nerve.