LncRNA H19 在食管癌组织中的表达 及其与细胞转移的关系

Clinical significance of LncRNA H19 in esophageal cancer and its relationship with cell metastasis

摘要:

目的 探究长链非编码RNA H19（LncRNA H19）在食管癌组织中表达的临床意义及其对人 食管癌细胞TE1 体外转移能力的影响。方法 选取2015 年6 月—2017 年3 月齐齐哈尔医学院附属第三医院 心胸外科收治的72 例食管癌患者的肿瘤组织及相应癌旁组织，采用实时荧光定量聚合酶链反应检测组织中 LncRNA H19 的表达情况，分析患者肿瘤组织中LncRNA H19 与性别、年龄、肿瘤组织分化程度及TNM 分 期的关系。实验组和对照组分别转染表达LncRNA H19 的慢病毒载体及空白载体于人食管癌细胞TE1 中， 采用Transwell 侵袭及迁移模型检测两组细胞的转移能力，Western blotting 检测两组细胞基质金属蛋白酶2 （MMP-2）及金属基质蛋白酶9（MMP-9）的表达。结果 食管癌组织中LncRNA H19 的表达高于癌旁组 织（P <0.05）；不同TNM 分期、淋巴结转移率及组织分化程度患者的LncRNA H19 表达水平有差异（P <0.05）。 实验组LncRNA H19 表达水平高于对照组（P <0.05）；实验组侵袭及迁移细胞数高于对照组（P <0.05）；实 验组MMP-2 及MMP-9 蛋白表达水平高于对照组（P<0.05）。结论 LncRNA H19 在食管癌组织中高表达， 且可通过上调MMP 的表达促进食管癌细胞的转移。

Abstract:

Objective To investigate the clinical significanceof expression of long non-coding RNA H19 (LncRNA H19) in esophageal carcinoma and its effect on the metastasis ability of human esophageal cancer cell line TE1 in vitro. Methods Totally 72 cases of esophageal carcinoma and the corresponding adjacent tissues were collected from department of cardio-thoracic surgery in our hospital from June 2015 to March 2017. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of LncRNA H19 in tissues, and analyze the relationship between the expression and gender, age, tumor differentiation, TNM stages. Lentivirus transfected the vector expressing LncRNA H19 and empty vector into human esophageal cancer cell line TE1, as the test group and control group. Transwell invasion and migration assay were used to detect the invasion and migration ability of both group cells. Western Bolt assay was used to detect the expression of matrix metalloproteinases-2 (MMP-2) and matrix metalloproteinase -9 (MMP-9). Results The expression of LncRNA H19 in esophageal carcinoma was significantly higher than that in adjacent tissue, and the difference was statistically significant (P < 0.05). Patients with high expression of LncRNA H19 in esophageal cancer tissue compared to those with low expression had lower degree of tissue differentiation and higher lymph node metastasis rate, and the difference was statistically significant (P < 0.05). The expression of LncRNA H19 in test group was significantly higher than that in control group cells, and the difference was statistically significant (P < 0.05). The numbers of invasive and migrating cells in test group were significantly higher than those in the control group, and the differences were statistically significant (P < 0.05). The expressions of MMP-2 and MMP-9 protein in test group were significantly higher than those in the control group, and the differences were statistically significant (P < 0.05). Conclusions LncRNA H19 is highly expressed in esophageal cancer tissues, and can promote the metastasis of esophageal cancer cells by up-regulating the expression of MMP.