MicroRNA-452 和microRNA-5100 在NSCLC 组织中的表达及其临床意义

The expressions and clinical significance of microRNA-452 and microRNA-5100 in the non-small cell lung cancer tissues

摘要:

目的 检测非小细胞肺癌（NSCLC）癌组织中microRNA-452（miR-452）和microRNA-5100 （miR-5100）的表达水平，并探究其临床意义。方法 采用实时聚合酶链反应（real-time PCR）检测94 例 NSCLC 患者的癌组织和癌旁正常组织中miR-452 和miR-5100 的表达水平，分析其与临床病理特征的关 系；采用Kaplan-Meier 生存曲线分析miR-452 和miR-5100 表达对患者术后5 年总生存率和生存时间的影 响。结果 NSCLC 癌组织中miR-452 相对表达量低于癌旁正常组织（P <0.05），NSCLC 癌组织中miR- 5100 相对表达量高于癌旁正常组织（P <0.05）。NSCLC 癌组织中，miR-452 高表达与肿瘤病理类型、肿 瘤分化程度、肿瘤大小及淋巴结转移有关（P <0.05）；miR-5100 高表达与肿瘤分化程度、TNM 分期有关 （P <0.05）。miR-452 高表达患者的术后5 年总生存率高于低表达患者（P <0.05），中位生存时间长于低表达 患者（P <0.05）；miR-5100 高表达患者的术后5 年总生存率低于低表达患者（P <0.05），中位生存时间短于 低表达患者（P <0.05）。结论 在NSCLC 癌组织中，miR-452 呈低表达、miR-5100 呈高表达，两者可能参 与NSCLC 肿瘤形成和恶性进展，影响患者预后。

Abstract:

Objective To detect the expression of micro RNA-452 (microRNA-452) and micro RNA- 5100 (microRNA-5100) in the cancer tissues of non-small cell lung cancer (NSCLC), and to explore the clinical significance. Methods The expression levels of microRNA-452 and microRNA-5100 in the cancer tissues and the adjacent normal tissues from 94 patients with NSCLC were determined by the real-time PCR assay. The correlations in the microRNA-452, microRNA-5100 expression and the clinicopathological characteristics were evaluated. The Kaplan-Meier survival curve was utilized to analyze the influences of expressions of microRNA-452 and microRNA-5100 on the 5-year survival rate and the survival time after operations. Results The real-time PCR results showed that in the NSCLC tissues, the expression of microRNA-452 was lower in the NSCLC tissues than that in the adjacent normal tissues, and the expression of microRNA-5100 was higher than that in the adjacent normal tissues, and there were both statistical significances (P < 0.05). In the NSCLC tissues, the high expression of microRNA-452 was correlated with tumor pathological type, the tumor differentiation degree, the tumor size and the lymph node metastasis (P < 0.05), and high expression of the microRNA-5100 was correlated with tumor differentiation degree and the TNM stages (P < 0.05). The Log Rank results suggested that the 5-year survival rate after operations of patients in the high-microRNA-452 group was higher than that in the low-microRNA-452 group (χ2= 8.324, P = 0.004), and the medium survival time of patients in the high-microRNA-452 group was longer than that in the low-microRNA-452 group (χ2= 7.318, P = 0.007); the 5-year survival rate after operations of patients in the high-microRNA-5100 group was lower than that in the low-microRNA-5100 group (χ2= 7.693, P = 0.006), and the medium survival time of patients in the high-microRNA-5100 group was shorter than that in the lowmicroRNA- 5100 group (χ2= 8.174, P = 0.004). Conclusions In the NSCLC tissues, the microRNA-452 is lowly expressed and the microRNA-5100 is highly expressed, which may exert synergistic action on the tumorigenesis and progression of NSCLC and influence the prognosis.