HMGB1 和HIF1a 在乳头状甲状腺癌中的 表达及意义

Expression and significance of HMGB1 and HIF1a in papillary thyroid carcinoma

摘要:

目的 探讨高迁移率族蛋白1（HMGB1）和缺氧诱导因子1a（HIF1a）在乳头状甲状腺癌（PTC） 中的表达及与临床病理特征的关系。方法 采用免疫组织化学法检测120 对PTC（肿瘤组）及其相应癌旁组 织（非癌组织组）中HMGB1 与HIF1a 的表达，比较两组HMGB1 与HIF1a 蛋白表达的差异，并分析其表达 水平与PTC 患者临床病理特征的关系。结果 PTC 组织中HMGB1 阳性表达率高于癌旁组织（P <0.05）； PTC 组织中HIF1a 阳性表达率高于癌旁组织（P <0.05）。PTC 组织中HMGB1 与HIF1a 的表达呈正相关 （P <0.05）。HIF1a 和HMGB1 在不同肿瘤直径、肿瘤数量、淋巴结转移情况及临床分期比较，差异有统计学 意义（P <0.05）结论 HMGB1、HIF1a 与PTC 增殖和转移相关，可能是PTC 发生、发展的重要分子，进一 步研究两者的关系可能为抑制肿瘤增殖、转移提供潜在的分子靶点。

Abstract:

Objective To investigate the expression of high mobility group protein 1 (HMGB1) and hypoxia inducible factor 1A (HIF1a) in papillary thyroid carcinoma and its significance. Methods The expression of HMGB1 and HIF1a in 120 patients with papillary thyroid carcinoma (tumor group) and its corresponding para-cancerous tissue (non-cancerous tissue group) was detected by immunohistochemistry. The difference between the expression of HMGB1 and HIF1a in the two groups was compared, and the relationship between the expression of the two groups and the pathological features of the patients with papillary thyroid carcinoma was analyzed. Results The positive expression rates of HMGB1 in PTC tissues and adjacent tissues were 65% and 35%, respectively. The difference was statistically significant by χ2 test (χ2= 8.067, P = 0.005), the positive expression rate of HMGB1 in PTC tissues was higher than that in adjacent tissues. The positive expression rates of HIF1a in PTC tissues and adjacent tissues were 59.2% and 40.8%, respectively. The positive expression rate of PTC was higher than that of adjacent tissues (χ2= 9.6,P = 0.002). Correlation analysis showed that HMGB1 was positively correlated with HIF1a expression in PTC tissues (r = 0.495, P = 0.014). The expression of HMGB1 in patients with different tumor sizes, tumor numbers, lymphatic metastases and clinical stages were statistical different (P < 0.05). Similarly, the high expression of HIF1a was also significantly different from patients with different tumor size, tumor number, lymphatic metastasis and clinical stage (P < 0.05). There were significant differences in tumor size, tumor number, lymphatic metastasis and clinical stage (P < 0.05). Conclusions HMGB1 and HIF1a are associated with proliferation and metastasis of papillary thyroid carcinoma. It may be an important molecule in the development of papillary thyroid carcinoma. The further study of the relationship may provide a potential molecular target for inhibiting tumor proliferation and metastasis.