右美托咪定在大鼠脑出血延迟低温治疗中的作用

Therapeutic effect of Dexmedetomidine on delayed hypothermia treatment of cerebral hemorrhage in rats

摘要:

目的??探讨右美托咪定在大鼠脑出血后延迟低温治疗中的作用。方法??选取健康雄性 SD 大鼠 95 只， 采用立体定向尾状核注射法复制大鼠脑出血模型，将 76 只复制成功的大鼠作为手术组，随机分为模型组、延迟 低温组、右美托咪定组、右美托咪定联合延迟低温组， 每组 19 只。余下的为假手术组 19 只给予手术处置但不注 射药物，手术组分别给予相应的治疗。对大鼠神经功能缺损（NDS）评分、脑组织水分含量、血肿周围脑组织 细胞凋亡指数、血清炎症细胞因子及脑损伤标志物水平进行检测。结果??治疗后， 与模型组比较， 各组大鼠 NDS 评分及脑组织水分含量较低，且延迟低温组和右美托咪定组均高于右美托咪定联合延迟低温组（ P <0.05） ； 各组 大鼠血肿周围脑组织细胞凋亡指数较低，且延迟低温组和右美托咪定组均大于右美托咪定联合延迟低温组（ P < 0.05） ； 各组大鼠血清肿瘤坏死因子 α、白细胞介素 8 水平较低，且延迟低温组和右美托咪定组均高于右美托 咪定联合延迟低温组（ P <0.05） ； 各组大鼠血清神经元烯醇化酶、S100β 蛋白水平较低，且延迟低温组和右 美托咪定组均高于右美托咪定联合延迟低温组（ P <0.05） 。结论??右美托咪定能够提高延迟低温治疗对大鼠脑 出血的疗效， 有效改善神经功能缺损， 减轻脑组织水肿， 抑制脑细胞凋亡， 减轻相关炎症反应和脑损伤程度。

Abstract:

Objective To explore the therapeutic effect of Dexmedetomidine on delayed hypothermia treatment of cerebral hemorrhage in rats. Methods Rat model of cerebral hemorrhage was established by stereotaxic caudate nucleus injection. Rats were randomly divided into model group, delayed hypothermia group, Dexmedetomidine group and Dexmedetomidine combined with delayed hypothermia group (n = 19). Rats in sham group received all procedure except hemorrhage. The neurological deficit (NDS) score, brain tissue water content, apoptosis rate in brain tissue, serum levels of inflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin -8 (IL-8) and brain injury markers were detected. Results Rats in model group experienced an increased levels of NDS score, brain tissue water content, apoptosis rat in brain tissue around hematoma, serum TNF-α as well as IL-8, serum neuronal enolase (NSE) and S100β protein (S100β) (P < 0.05) when compared with those in sham group (P < 0.05). Treatment of Dexmedetomidine or delayed hypothermia induced a significant decrease of the mentioned indexes compared with model group (P < 0.05) while therapeutic effect of Dexmedetomidine plus delayed hypothermia was synergistic compared to Dexmedetomidine or delayed hypothermia only group (P < 0.05). Conclusions Dexmedetomidine enhances the therapeutic effect of delayed hypothermia therapy in rats model of cerebral hemorrhage.