儿茶酚胺抑素在自发性高血压大鼠交感神经活化中的作用

Role of catestatin in sympathetic activation in spontaneously hypertensive rats

摘要:

目的??探讨儿茶酚胺抑素（catestatin）在高血压发展过程中的变化规律及其对交感神经活性的作用。 方法??以自发性高血压大鼠（SHR） （SHR 组）及其相应的正常大鼠（WKY） （WKY 组）作为研究对象。在大 鼠不同的发育阶段（6、12 和 16 周龄）检测心率、血压及血浆 catestatin 水平。以心率作为交感神经活性的标 志。酶联免疫吸附测定 catestatin 的浓度，尾套法检测大鼠的血压及心率。结果??SHR 组各个周龄的血压、心率 均高于相应周龄的 WKY 组大鼠。SHR 组血浆 catestatin 浓度高于 WKY 组（ P <0.05） ，随着周龄的增加，SHR 组血浆 catestatin 浓度逐渐升高， 6 周龄为（1.60±0.54）ng/ml， 12 周龄为（1.76±0.26）ng/ml， 16 周龄为（3.03± 0.57）ng/ml。而 WKY 组大鼠则保持不变，6 周龄为（1.10±0.28）ng/ml，12 周龄为（1.05±0.21）ng/ml，16 周 龄为（1.17±0.12）ng/ml，两组血浆 catestatin 的浓度变化趋势有差异（ P <0.05） 。12 周 SHR 组大鼠尾静脉注射 catestatin，30?min 后心率由（417.6±20.9）次 /min 降到（378.1±15.6）次 /min（ P <0.05） ； 但血压未下降。结论? catestatin 具有抑制心率的作用， 可能具有代偿性抑制交感活性的作用。

Abstract:

Objective To investigate the changes of catecholamine catestatin in the development of hypertension and its effect on sympathetic nerve activity. Methods Spontaneously hypertensive rats (SHR) and their littermates Wistar-Kyoto rats (WKY) were used in this study. Heart rate, blood pressure, and plasma catestatin levels were measured at different developmental stages (6, 12, and 16 weeks of age) in rats. Heart rate is used as a marker of sympathetic activity. The concentration of catestatin was detected by enzyme-linked immunosorbent assay, and the blood pressure and heart rate of the rats were detected by tail-clamp method. Results The blood pressure and heart rate of SHR in each age group were higher than those of WKY mice of corresponding age. The plasma catestatin concentration in SHR was higher than WKY (P < 0.05). The plasma catestatin concentration gradually increased with the increase of week age, (1.60 ± 0.54) ng/ml at 6 weeks, (1.76 ± 0.26) ng/ml at 12 weeks and (3.03 ± 0.57) ng/ml at 16 weeks of age.The WKY rats remained unchanged at (1.10 ± 0.28) ng/ml at 6 weeks of age, (1.05 ± 0.21) ng/ml at 12 weeks of age, and (1.17 ± 0.12) ng/ml at 16 weeks of age. There was a difference in the trend of change (P < 0.05). After 12 weeks of SHR, cestastatin was injected into the tail vein. After 30 minutes, the heart rate decreased from (417.6 ± 20.9) times/min to (378.1 ± 15.6) times/min (P < 0.05), but the blood pressure did not decrease significantly before injection. conclusions Catestatin can inhibit the heart rate of SHR, and its gradual increase in SHR plasma may have compensatory inhibition of sympathetic activity.