高效液相色谱法同时检测复方紫草油微乳主药成分含量的研究

Simultaneous determination of main components in compound Radix arnebiae oil microemulsion by high performance liquid chromatography

摘要:

目的??建立高效液相色谱法同时检测复方紫草油微乳中黄芩苷、欧前胡素、紫草素的含量。方法 采 用色谱柱以 0.1% 磷酸溶液（A）- 乙腈（B）为流动相（梯度洗脱： 0 ～ 12 min， 30%?B； 12 ～ 30 min， 30% ～ 54%?B； 30 ～ 45?min，54%?B ； 45 ～ 60?min，54% ～ 85%?B） ； 流速 1.0 ml/min ； 检测波长 254 nm ； 柱温 30℃。结果??黄芩 苷、欧前胡素及紫草素分别在 2.073 ～ 16.589、0.023 ～ 0.185、0.081 ～ 0.645 μg 范围内峰面积呈良好的线性关 系（ r ≥ 0.9999） ； 平均加样回收率分别 95.79%、99.04% 和 97.32%，RSD 分别为 3.23%、3.07% 和 2.71%。结论? 该方法准确、灵敏、专属性强， 为复方紫草油微乳提供多指标含量检测的质量控制方法。

Abstract:

Objective To establish an high performance liquid chromatography (HPLC) method for measurement of Baicalin, Imperatorin and Shikonin in microemulsion of radix arnebiae oil. Methods The method was conducted on an Diamonsil C18 (5 μm, 250 mm×4.6 mm) with 0.1% phosphoricacid (A) – cetonitril (B) as mobile phase (gradient elution: 0-12 min, 30% B; 12-30 min, 30%-54% B; 30-45 min, 54% B; 45-60 min, 54%-85% B), low rate was 1.0 ml/min, detecting at 254 nm, column temperature 30℃. Results The linear ranges of Baicalin, Imperatorin and Shikonin were 2.073-16.589 μg, 0.023-0.18 μg and 0.081-0.645 μg, respectively, r ≥ 0.9999. The average recoveries (n = 6) of the three chemicals were 95.79%, 99.04%, 97.32%, respectively. RSD of Baicalin, Imperatorin and Shikonin was 3.23%, 3.07%, 2.71%, respectively. Conclusions HPLC is accurate, sensitive and specific for quality control of microemulsion of radix arnebiae oil.