SBHFRT 和CFRT 技术对NSCLC 组织中 淋巴细胞和细胞因子的影响

Effects of SBHFRT and CFRT on lymphocytes and cytokines in NSCLC tissues

摘要:

目的 探讨单次近距离大分割放疗（SBHFRT）和常规分割放疗（CFRT）对非小细胞肺癌 （NSCLC）组织中淋巴细胞和细胞因子的影响。方法 将NSCLC 移植瘤模型小鼠随机分为对照组、CFRT 组及SBHFRT 组，每组6 只小鼠。计算放疗结束后第14 天肿瘤体积和肿瘤生长抑制率（TGIR），以18F-FDG micro PET-CT 检测肿瘤标准摄取值（SUV），评估肿瘤代谢活性；免疫组织化学SP 法和酶联免疫吸附测定 法检测肿瘤组织中CD4+、CD8+ 及Foxp3+ T 淋巴细胞的表达和白细胞介素10（IL-10）、白细胞介素12（IL- 12）及γ 干扰素（INF-γ）的含量。结果 SBHFRT 组CD4+、CD8+ T 淋巴细胞表达高于CFRT 组和对照 组，而Foxp3+ T 淋巴细胞表达低于CFRT 组和对照组（P <0.05）；SBHFRT 组IL-12 和INF-γ 含量高于 CFRT 组和对照组，而IL-10 含量低于CFRT 组和对照组（P <0.05）；SBHFRT 组肿瘤体积、肿瘤SUV 值 低于CFRT 组和对照组（P <0.05）；SBHFRT 组TGIR 高于CFRT 组（P <0.05）。结论 SBHFRT 相对于 CFRT 能增加NSCLC 肿瘤组织中抗肿瘤淋巴细胞表达和具有肿瘤杀伤作用的细胞因子含量，更能抑制肿瘤 生长，降低肿瘤代谢活性。

Abstract:

Objective To investigate the influence of single brachytherapy hypofractionated radiotherapy (SBHFRT) and conventional fractionation radiotherapy (CFRT) on lymphocytes and cytokines in non-small cell lung cancer (NSCLC). Methods The NSCLC model mice were randomly divided into control group, conventional fractionation radiotherapy (CFRT) group and single brachytherapy hypofractionated radiotherapy (SBHFRT) group. The tumor volume of mice in each group and tumor growth inhibition rate (TGIR) of treatment groups were calculated on the 14th day after the end of treatment. The tumor metabolism was assessed by micro 18F-FDG PET-CT imaging. The Immunohistochemistry SP method was used to detect the expression of CD4+, CD8+ and Foxp3+ T lymphocytes in tumor tissues of the mice in each group and the content of interleukin-10 (IL-10), interleukin-12 (IL-12) and interferon-γ (INF-γ) in tumor tissues were measured by enzyme-linked immunosorbent assay (ELISA). Results The expressions of CD4+ and CD8+ T lymphocytes in the SBHFRT group were higher than those in the CFRT group and the control group (P < 0.05), while the expression of Foxp3+ T lymphocytes in the SBHFRT group was lower than that in the CFRT group and the control group (P < 0.05). The contents of IL-12 and INF-γ in the SBHFRT group were higher than those in the CFRT group and the control group (P < 0.05), while the content of IL-10 in the SBHFRT group was lower than that in the CFRT group and the control group (P < 0.05). The tumor volume and standard uptake value (SUV) in the SBHFRT group were lower than those of the CFRT group and the control group (P < 0.05). Compared with the CFRT group, the TGIR of SBHFRT group was higher (P < 0.05). Conclusions Compared with CFRT, SBHFRT can increase the content of antitumor lymphocytes and cytokines to inhibit tumor growth and reduce tumor activity more significantly.