牡丹皮、赤芍配伍对活血化瘀疗效及有效成分的影响

Effect of Moutan cortex and Paeoniae rubra radix on coagulation and their active ingredients

摘要:

目的 探讨牡丹皮、赤芍配伍对家兔血小板聚集、凝血酶时间、血栓溶解、全血凝块溶解的影响。并考察两药配伍对没食子酸、芍药苷、丹皮酚的影响。方法 比浊法测定血小板聚集抑制率和凝血酶时间，重量法测定血栓溶解率和全血凝块溶解率，HPLC法测定主要成分的含量。结果 对照组的家兔体外血小板最大聚集率为42.62%，血浆凝血酶时间13.45?s，给予不同药液后，各组血小板最大聚集率均下降（P?<0.05），血浆凝血酶时间均上升（P?<0.05），其中，合煎组药效最好，其体外血小板聚集率为20.77%，凝血酶时间为21.01?s。同时24?h内，两药合煎与单煎再混合的血栓溶解率及全血凝块溶解率高于单煎牡丹皮及单煎赤芍。HPLC分析结果显示，两药合煎后与单煎再混合比较，芍药苷含量增加（P?<0.05）、没食子酸含量几乎持平，差异无统计学意义（P?>0.05）。结论 牡丹皮、赤芍合煎，能够促进有效成分的溶出，提高活血化瘀疗效，具有配伍合理性，为临床合理用药提供科学依据。

Abstract:

Objective To evaluate the effects of Moutan cortex and Paeoniae rubra radix on coagulation and potential casual ingredients. Methods Platelet aggregation was determined by turbidimetric method. Thrombolytic rate and the rate of whole blood clot were determined by gravity method. The content of each plant was determined by HPLC. Results The maximum platelet aggregation rate of rabbits in the control group was 42.62%, and the plasma thrombin time was 13.45?s. After different drug solutions, the maximum aggregation rate of platelets decreased (P??0.05). Conclusions Joint application of Moutan cortex and Paeoniae rubra radix exerts better anti-coagulation effect by enhancing dissolution of casual ingredients, which has rational compatibility, and provides scientific basis for clinical rational drug use.