MicroRNA-134对宫颈癌细胞增殖和凋亡的影响

Role of MicroRNA-134 in proliferation capability of ovarian cancer

摘要:

目的 探讨人宫颈癌组织中microRNA-134（miR-134）的表达及对宫颈癌细胞增殖和凋亡的影响。方法 PCR法检测宫颈癌组织中miR-134表达水平；在HeLa细胞内过表达miR-134，通过平板克隆形成试验检测细胞增殖，通过流式细胞仪检测细胞周期及细胞凋亡；PCR及western blotting检测miR-134潜在靶点CCND1表达变化。结果 宫颈癌组织中miR-134的表达水平较正常宫颈黏膜组织低（P?<0.05）；过表达miR-134能够抑制宫颈癌HeLa细胞增殖（P?<0.05）、阻滞细胞周期进展（P?<0.05），并促进细胞凋亡（P?< 0.05）；过表达miR-134后抑制HeLa细胞中CCND1 mRNA及蛋白的表达水平（P?<0.05）。结论 宫颈癌组织中miR-134表达水平降低，过表达miR-134能下调CCND1的表达发挥抗癌细胞生长作用。

Abstract:

Objective To investigate the role of MicroRNA-134 on proliferation and apoptosis of ovarian cancer. Methods The expression of MicroRNA-134 in ovarian cancer was measured by PCR. Clone formation assay and flow cytometry were performed to detect cell proliferation, cell cycle and apoptosis. PCR and Western blots were utilized for measurement of CCND1. Results MicroRNA-134 expression was down-regulated in ovarian cancer tissues (P?