microRNA-29a在结直肠癌组织中的表达及意义

Expression and significance of miRNA-29a in colorectal cancer

摘要:

目的 探讨microRNA-29a（miR-29a）在结直肠癌组织中的表达，以及miR-29a反义寡核苷酸对结直肠癌细胞增殖和侵袭的影响。方法 选取行手术治疗的初诊结直肠癌患者195例，利用实时荧光定量聚合酶链反应检测结直肠癌和癌旁组织中miR-29a表达，培养人结直肠癌LoVo细胞，随机分为ASO-miR-29a组、ASO-对照序列组和空白对照组，检测各组细胞中miR-29a表达，MTT法检测各组细胞增殖能力，Transwell小室检测各组细胞迁移和侵袭能力。结果 结直肠癌组织miR-29a相对表达量为（1.93±0.19），癌旁组织为（1.26±0.12），两者比较差异有统计学意义（P?<0.05）；结直肠癌组织中miR-29a相对表达量与TNM分期和淋巴结转移有关（P?<0.05），多元线性回归分析结果显示，TNM分期和淋巴结转移是影响结直肠癌组织中miR-29a表达的因素（P?<0.05）；ASO-miR-29a组细胞中miR-29a相对表达量低于ASO-对照序列组和空白对照组（P?<0.05）；MTT实验结果显示，ASO-miR-29a组与ASO-对照序列组和空白对照组相比吸光度值降低（P?<0.05）；与ASO-对照序列组和空白对照组比较，ASO-miR-29a 组迁移细胞数和侵袭细胞数均减少（P?<0.05）。结论 miR-29a在结直肠癌组织中呈高表达，参与结直肠癌发生、进展过程，特异性抑制结直肠癌细胞中miR-29a表达可减少细胞增殖，抑制细胞迁移和侵袭能力。

Abstract:

Objective To investigate the expression of miRNA-29a in colorectal cancer, and the effect of miRNA-29a on proliferation and invasion of colorectal cancer cells. Methods A total of 195 cases of colorectal cancer were involved in this study. The expression of miRNA-29a in colorectal cancer tissue and adjacent normal tissue was detected by real-time fluorescence quantitative PCR. Human colorectal cancer LoVo cell line was cultured. All cells were randomly divided into ASO-miRNA-29a group, ASO-control sequence group and blank control group. MTT and Transwell assay were performed to detect the cell proliferation and invasion, respectively. Results The relative expression level of miRNA-29a in the colorectal cancer tissues was increased when compared with adjacent tissues was [(1.93?±?0.19) vs (1.26?±?0.12), P?