MicroRNA-122-5p与MicroRNA-199a-5p在EMS患者血清的表达及临床意义

Expression and clicnial significance of miR-122-5p and miR-199a-5p in serum of patients with endometriosis

摘要:

目的 探讨microRNA-122-5p（miR-122-5p）与microRNA-199a-5p（miR-199a-5p）在子宫内膜异位症（EMS）患者血清的表达及临床意义。方法 前瞻性设计，随机选取2016年1月—2017年12月华北石油管理局总医院诊治的EMS患者90例（EMS组）及同期不孕症检查的患者70例（对照组）为研究对象。分别采用实时荧光定量聚合酶链反应（qRT-PCR）及酶联免疫吸附试验（ELISA）检测两组人群血清的miR-122-5p和miR-199a-5p的表达及白细胞介素-6（IL-6）的表达，并分析其与临床特征的相关性。构建受试者操作特征（ROC）曲线。结果 EMS组患者的血清及腹腔液中IL-6、miR-122-5p、miR-199a-5p的表达与对照组比较，差异有统计学意义（P?<0.05），EMS组均增高。miR-122-5p的表达与IL-6水平（r?=0.578，P?<0.05）、miR-199a-5p表达（r?=0.721，P?<0.05）呈正相关。miR-199a-5p的表达与IL-6水平（r?=0.562，P?<0.05）呈正相关。miR-122-5p和miR-199a-5p检测EMS的敏感性分别为94.7%和92.1%，特异性分别为90.5%和88.6%。结论 血清miR-122-5p和miR-199a-5p在EMS患者中的表达增加，miR-122-5p与miR-199a-5p有成为诊断子宫内膜异位症的生物标志物的潜力。

Abstract:

Objective To explore the expression and clinical significance of miR-122-5p and miR-199a-5p in patients with endometriosis (EMS). Methods Ninety patients with endometriosis and patients receiving infertility examination were involved in this study. Quantitative RT-PCR and enzyme linked immunosorbent assay were performed to detect the expression levels of miR-122-5p, miR-199a-5p and interleukin-6. Clinical relationships of the mentioned index with clinical features were analyzed. Results The serum levels of IL-6, miR-122-5p, and miR-199a-5p were significantly higher in endometriosis patients than in control group (P?