高压氧对糖尿病大鼠认知功能 及NGF mRNA 表达的影响

Effects of hyperbaric oxygen therapy on cognitive function and nerve growth factor mRNA expression of diabetes rats

摘要:

目的 探讨高压氧疗法对2 型糖尿病（T2DM）大鼠认知功能及脑组织神经生长因子microRNA （NGF mRNA）表达的影响。方法 将48 只成年健康SD 大鼠按随机数字表法分为空白对照组（12 只）、 T2DM 模型组（36 只）。模型复制成功后，T2DM 模型组大鼠再次按随机数字表法分为非干预A 组（6 只）、 非干预B 组（12 只），以及高压氧A 组（6 只）、高压氧B 组（12 只）。10 d 后采用Morris 水迷宫实验检测 非干预A 组、高压氧A 组认知功能，空白对照组、非干预B 组、高压氧B 组随机选取6 只大鼠脑组织检测 NGF mRNA。高压氧A、B 组于注射15 d 后开始高压氧干预，1 次/d，20 d 后各组再次分别检测认知功能 及NGF mRNA。结果 与非干预A 组比较，高压氧A 组逃避潜伏期缩短（P <0.05），穿越虚拟平台次数增 多（P <0.05）；与非干预B 组比较，高压氧B 组脑组织NGF mRNA 表达水平升高（P <0.05）。结论 高压氧 治疗可显著改善T2DM 大鼠认知功能，提升脑组织NGF mRNA 表达水平，提示高压氧治疗可有效改善早期 T2DM 患者认知功能，其机制可能与高压氧增强脑组织NGF mRNA 表达有关。

Abstract:

Objective To explore the effects of hyperbaric oxygen therapy on cognitive function and expression of nerve growth factor mRNA (NGF mRNA) in brain tissue of type 2 diabetes mellitus (T2DM) rats. Methods Totally 48 healthy adult SD rats were randomly divided into two groups: blank control group (n = 12) and T2DM model group (n = 36). After 4 weeks of a high -sugar high-fat diet, the rats in T2DM model group were intraperitoneally injected with streptozotocin (35mg/kg) only once. Three days later, the random blood glucose (RBG) was measured. If the RBG was more than 16.7mmol/L, we think those rats were the type 2 diabetic rats. Then those rats were randomly divided into four groups by using the random number table method: intervention group A (n = 6), non-intervention group B (n = 12), hyperbaric oxygen therapy group A (n = 6) and hyperbaric oxygen therapy group B (n = 12). 10 days after the injection, the Morris water maze was used to test the cognitive function of non-intervention A group and hyperbaric oxygen treatment group A. 6 rats were randomly selected from blank control group, non-intervention B group and hyperbaric oxygen treatment group B, and their brain were used for the NGF mRNA test. 15 days after the injection, rats in hyperbaric oxygen group A and B were given hyperbaric oxygen intervention (1 times /d). 20 days after treatment, the rats in each group were tested for their cognitive function and NGF mRNA. Results Compared with non-intervention group A, the latency period of escape in hyperbaric oxygen group A was significantly shortened (P < 0.05) and the number of times the rats traversed the virtual platform was significantly increased (P < 0.05). Compared with non-intervention group B, the expression level of NGF mRNA in brain tissue of hyperbaric oxygen treatment group B was increased (P < 0.05). Conclusions Hyperbaric oxygen therapy can significantly improve the cognitive function of T2DM rats and improve the expression level of NGF mRNA in brain tissue. The results suggest that hyperbaric oxygen therapy can improve the cognitive function in early T2DM patients effectively, and the mechanism may be related to the effect of hyperbaric oxygen on NGF mRNA expression in brain tissue.