自然流产患者的卵泡液代谢标志物研究

Study of follicular fluid metabolic markers in patients with spontaneous abortion based on follicular fluid metabonomics

摘要:

目的 探讨与自然流产发生相关的卵泡液（FF）代谢标志物，以进一步科学阐释自然流产的发 生机制。方法 利用超高效液相色谱- 高分辨质谱技术对因输卵管因素行体外受精- 胚胎移植（IVF-ET） 后自然流产患者即流产组（40 例）及分娩者即对照组（45 例）的FF 进行代谢组学分析。结果 流产组和对 照组FF 共32 种差异代谢物，主要有氨基酸类、胆固醇类、维生素类、脂肪酸类、胆酸类、溶血卵磷脂类及 其他7 大类，其中上调的有Dehydroepiandrosterone sulfate，LysoPC（16 ∶ 0），Palmitoylglycerophosphocholine 等8 种，下调的有L-valine，6-Oxohexanoate，L-Phenylalanine，2-Oxoglutarate，Linoleate 等24 种。结论 本 研究鉴定的32 种差异代谢物与自然流产相关，为自然流产提供可能的诊断依据和治疗靶点，从FF 代谢组学 层面为阐释自然流产发生机制提供科学依据。

Abstract:

Objective To investigate the follicular fluid metabolic markers associated with spontaneous abortion to further elucidate the mechanism of spontaneous abortion. Methods Follicular fluid metabonomics analysis was performed on abortion group (n = 40) and control group (n = 45) who underwent in vitro fertilization-embryo transfer (IVF-ET) by ultra-high performance liquid chromatography-high resolution mass spectrometry. Results There were 32 kinds of different follicular fluid metabolites between abortion group and control group, including amino acids, cholesterol, vitamins, fatty acids, bile acids, lysolecithin and others. Dehydroepiandrosterone sulfate, LysoPC (16: 0), Palmitoylglycerophosphocholine and other 5 metabolites were up-regulated, and 24 species were down-regulated, including L-valine, 6-Oxohexanoate, L-Phenylalanine, 2-Oxoglutarate, Linoleate and so on. Conclusions The 32 differential metabolites identified in this study are related to spontaneous abortion and provide a possible diagnostic basis and therapeutic target for spontaneous abortion. It provides a scientific basis for elucidating the mechanism of spontaneous abortion from the perspective of follicular fluid metabonomics.