MicroRNA-7 对膀胱癌诊断和复发的作用机制研究

Mechanism of miR-7 in the diagnosis and recurrence of bladder cancer patients

摘要:

目的 探讨microRNA-7（miR-7）对膀胱癌患者诊断和复发的作用及意义。方法 选取2014 年12 月—2015 年12 月绵阳市第三人民医院收治的90 例膀胱癌（膀胱癌组）、30 例正常（正常组）、30 例 泌尿结石（泌尿结石组）及30 例泌尿感染患者（泌尿感染组）病例资料，采集患者血清和晨尿，采用实时聚 合酶链反应检测患者血清和晨尿miR-7 含量，分析其诊断价值，以及与肿瘤预后及复发的关系。结果 与 正常组比较，膀胱癌组血清miR-7 mRNA 表达下降（P <0.05）；正常组、泌尿结石组及泌尿感染组尿沉渣 miR-7 mRNA 表达比较，差异无统计学意义（P >0.05）；与正常组比较，膀胱癌组尿沉渣miR-7 mRNA 表 达下降（P <0.05）；膀胱癌组尿沉渣miR-7 诊断价值优于血清miR-7（P <0.05）；随着膀胱癌患者TNM 分 期加重，膀胱癌患者血清及尿沉渣miR-7 mRNA 下降（P <0.05）；对90 膀胱癌患者进行术后随访，随访时 间（1.36±0.59）年，其中25 例出现术后复发，复发性膀胱癌患者血清及尿沉渣miR-7mRNA 表达较未复发 膀胱癌患者下降（P <0.05）。结论 血清和尿沉渣miR-7 可作为膀胱癌诊断、预后及随访的生物学肿瘤标 志物。

Abstract:

Objective To investigate the effect and significance of miR-7 in the diagnosis and recurrence of bladder cancer patients. Methods Ninety patients with bladder cancer and 90 non-tumor subjects (30 in normal group, 30 in urinary stone group, 30 in urinary infection group)were enrolled in our hospital from 2014.12 to 2015.12. Serum and morning urine were detected by Real-Time PCR. The levels of miR-7 were analyzed for diagnostic value and relationship between prognosis and recurrence. Results Compared with the normal group, the expression of serum miR-7 mRNA in bladder cancer patients was decreased (P < 0.05). There was no difference in the expression of miR-7 mRNA in urine sediments among normal group, urinary stone group and urinary infection group (P > 0.05). Compared with the normal group, the expression of miR-7 mRNA in urine sediments of bladder cancer patients was decreased (P < 0.05). The diagnostic value of urine sediment miR-7 was greater than that of serum miR-7 (P < 0.05). With the aggravation of TNM staging in bladder cancer patients, the expression of miR-7 mRNA in serum and urine sediment of bladder cancer patients decreased (P < 0.05). Postoperative follow-up was performed on 90 patients with bladder cancer. The follow-up time was (1.36±0.59) years, and 25 of them had postoperative recurrence. The expression of miR-7 mRNA in serum and urine sediment of patients with recurrent bladder cancer was decreased (P < 0.05). Conclusions Serum and urinary sediment miR-7 can be used as a biological tumor marker for the diagnosis, prognosis and follow-up of bladder cancer.