血清TXNIP、Trx、PKCβ1 水平与2 型糖尿病患者 颈动脉内中膜厚度的相关性分析

Correlation between expression of serum TXNIP, Trx and PKCβ1 and carotid intima-media thickness in type 2 diabetic patients

摘要:

目的 探讨2 型糖尿病（T2DM）患者血清中硫氧还蛋白相互作用蛋白（TXNIP）、硫氧还蛋白（Trk） 与蛋白激酶Cβ1（PKCβ1）水平与颈动脉内中膜厚度（IMT）的相关性。方法 选取2015 年4 月—2017 年 5 月承德市中心医院就诊的114 例T2DM 患者作为观察组，并根据是否存在颈动脉内中膜增厚分为内中膜增 厚亚组（83 例）和内中膜非增厚亚组（31 例），另选择同期114 例健康体检者作为对照组。比较3 组性别组成、 年龄、体重指数（BMI）、收缩压（SBP）、舒张压（DBP）、吸烟情况、是否合并高血压、空腹血糖（FPG）、 甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、总胆固醇（TC）、糖化血红蛋白水平（HbA1c）、 TXNIP、Trx、PKCβ1 及颈动脉IMT 值的差异。通过Logistic 回归分析寻找影响颈动脉IMT 的独立危险因 素，通过Spearman 相关性分析进一步确认TXNIP、Trx 及PKCβ1 与颈动脉IMT 的相关性。结果 观察组 FPG、HbA1c、TXNIP、Trx 及PKCβ1 颈动脉IMT 值均高于对照组（P <0.05），内中膜增厚亚组各指标均 高于内中膜非增厚亚组（P <0.05）；多因素Logistic 回归分析显示，HbA1c[Ol ^ R=1.242，95% CI（1.104，1.457）]、 TXNIP[Ol ^ R=1.794，95% CI（1.382，2.351）]、Trx[Ol ^ R=1.537，95% CI（1.343，2.185）] 及PKCβ1[Ol ^ R=1.321， 95% CI（1.132，1.646）] 是颈动脉IMT 的独立危险因素（P <0.05）；同时TXNIP、Trx 及PK Cβ1 与颈动脉 IMT 值呈正相关（r s =0.586、0.368 及0.413，P =0.013、0.029 及0.032）。结论 TXNIP、Trx 及PKCβ1 是颈 动脉IMT 的独立危险因素，且均呈正相关。

Abstract:

Objective To investigate the relationship between expression of serum TXNIP, Trx and PKCβ1 and carotid intima-media thickness (IMT) in patients with type 2 diabetes mellitus (T2DM). Methods A total of 114 T2DM patients treated in our hospital from April 2015 to May 2017 were included in the observation group. According to the presence or absence of carotid intimal thickening, they were divided into two groups: intimal thickening sub-group (83 cases) and non-thickening intima sub-group (31 cases), and the other 114 cases of healthy subjects in the same period were included in the control group. The differences of sex composition, age, body mass index, systolic blood pressure, diastolic blood pressure, smoking status, hypertension, fasting blood glucose, triglycerides, low density lipoprotein, high density lipoprotein, total cholesterol, glycosylated hemoglobin (HbA1c), TXNIP, Trx, PKCβ1 and carotid IMT were compared. Logistic regression analysis was used to identify independent risk factors affecting carotid IMT. The correlation between TXNIP, Trx and PKCβ1 and carotid IMT was further confirmed by Spearman correlation analysis. Results For the fasting blood glucose, HbA1c, TXNIP, Trx, PKCβ1and carotid IMT value, the indicators of the observation group were higher than those of the control group (P < 0.05), and those of the intimal thickening sub-group were higher than those of non-thickening intimal sub-group (P < 0.05). Multivariate Logistic regression analysis showed that HbA1c [Ol^R=1.242, 95% CI (1.104, 1.457)], TXNIP [Ol^R=1.794, 95% CI (1.382, 2.351)], Trx [Ol^R=1.537, 95% CI (1.343, 2.185)] and PKCβ1 [Ol^R=1.321, 95% CI (1.132, 1.646)] were independent risk factors of carotid artery IMT. At the same time, TXNIP, Trx and PKCβ1 showed positive correlation with carotid IMT (r = 0.586, 0.368, 0.413, P = 0.013, 0.029, 0.032). Conclusions TXNIP, Trx and PKCβ1 are independent risk factors of carotid artery IMT, and have positive correlation with the latter.