Urantide对动脉粥样硬化大鼠心脏UⅡ和GPCR14基因与蛋白表达的影响*

Effect of Urantide on expression of U II and GPCR14 in atherosclerotic heart*

摘要:

目的 观察Urantide对动脉粥样硬化（AS）大鼠心脏UⅡ和GPCR14基因与蛋白表达的影响。方法 采用腹腔注射维生素D3及高脂饲料喂养复制大鼠AS模型，随机分为正常组、AS组、辛伐他汀组、Urantide组（3、7和14?d组）。应用免疫组织化学、实时荧光定量聚合酶链反应（qRT-PCR）、Western blotting检测大鼠心脏UⅡ、GPCR14基因和蛋白的表达水平。结果 与正常组比较，AS组大鼠心脏血管及病变部位UⅡ及GPCR14阳性表达增加（P?<0.05），UⅡ及GPCR14基因与蛋白质的表达水平升高（P?<0.05）。与AS组比较，Urantide各给药组大鼠心脏血管及病变部位的UⅡ及GPCR14阳性表达减少（P?<0.05），UⅡ及GPCR14基因与蛋白质的表达水平降低（P?<0.05）。结论 Urantide对AS大鼠的心脏具有保护作用。

Abstract:

Objective To investigate the effect of Urantide on the expression of U II and GPCR14 gene and protein in atherosclerotic heart. Methods A rat model of atherosclerosis (AS) was established by high-fat diet plus intraperitoneal injection of VD3. Rats were randomly divided into Normal group, AS group, Simvastatin group and Urantide group. Expression of U II and GPCR14 gene and protein in rat heart was measured by immunohistochemistry, qRT-PCR and Western blotting. Results Positive staining of U II and GPCR14 in AS heart tissue were increased significantly when compared with normal group (P?