皮损间充质干细胞中相关细胞因子异常分泌对银屑病的临床意义

Clinical significance of abnormal secretion of cytokines in lesional mesenchymal stem cells of psoriatic patients

摘要:

目的 探讨表皮生长因子（EGF）、转化生长因子β（TGF-β）及干细胞因子（SCF）在银屑病发生、发展中的作用。方法 体外分离培养银屑病患者（观察组）与健康皮肤患者（对照组）皮肤间充质干细胞，采用流式细胞仪检测鉴定皮肤间充质干细胞，显微镜下观察成脂及成骨诱导分化后的皮肤间充质干细胞，采用ELISA检测皮肤间充质干细胞中EGF、TGF-β1及SCF的水平。结果 观察组与对照组的皮肤间充质干细胞显微镜下形态相似；观察组表面抗原CD73、CD44、CD90、CD105、以及CD29表达阳性，而HLA-DR、CD34以及CD45表达阴性；成脂及成骨诱导分化能力相似；观察组皮肤间充质干细胞中EGF、TGF-β1及SCF的水平与对照组比较，差异有统计学意义（P?<0.05），观察组皮损处皮肤间充质干细胞中EGF及SCF水平表达升高，而TGF-β1水平降低。结论 银屑病患者皮损间充质干细胞EGF、TGF-β1及SCF分泌异常。

Abstract:

Objective To investigate the levels of epidermal growth factor (EGF), transforming growth factor β1 (TGF-β1) and stem cells factor (SCF) in mesenchyma stem cell in lesion of psoriasis patients and potential clinical significance. Methods Cultivation and isolation of mesenchyma stem cells were collected from the skin of psoriatic patients and healthy volunteers. The identification of mesenchyma stem cells was performed with flow cytometry. Morphological characteristics of mesenchyma stem cell were observed by the microscope after adipogenic and osteoblast differentiation. The levels of EGF, TGF-β1 and SCF in mesenchyma stem cells were tested by ELISA. Results Similar morphology of mesenchyma stem cell from psoriatic group and healthy control group were observed. Mesenchyma stem cells from psoriatic group possessed positive expression of CD73, CD44, CD90, CD105 and CD29 while no expressions of HLA-DR, CD34 and CD45 were detected. Adipogenic and osteoblast differentiation capabilities were similar. Mesenchyma stem cells from psoriatic group indicated increased levels of EGF and SCF and decreased levels of TGF-β1 compared with those from healthy controls. Conclusions The abnormal secretion of EGF, TGF-β1 and SCF in mesenchyma stem cells may be involved in onset and progression of psoriasis.