Objective To investigate the advantages of 3D total laparoscopic hemicolectomy of right colon carcinoma with complete mesocolic excision (CME). Methods A total of 108 cases of right colon carcinoma were involved in this study. Patients were randomly divided into 2D group (n?=?62) and 3D group (n?=?46), all of which received laparoscopic hemicolectomy of right colon carcinoma with CME. Results The operation time and intraoperative blood loss during the second step (suture) in the 3D group significantly decreased compared with those in 2D group (P?0.05). Difficulty degree in depth perception and endovascular operation (suture and knotting) 3D group was weakened obviously when compared with that in the 2D group (P?0.05). Conclusions Laparoscopic 3D system provides a clear picture of operation, reduces the operation time and blood loss in laparoscopic hemicolectomy of right colon carcinoma with CME.