血清microRNA-4286 表达与胃癌临床 病理特征的关系及其临床意义

Correlation between serum miR-4286 expression and clinicopathological characteristics of gastric cancer and its clinical significance

摘要:

目的 探讨胃癌患者血清microRNA-4286（miR-4286）的表达水平与胃癌临床病理特征的关 系，分析其对胃癌的临床诊断效能及意义。方法 通过实时荧光定量聚合酶链反应检测健康体检者（对照组） 和胃癌患者（胃癌组）血清miR-4286 的表达，分析miR-4286 的表达量与胃癌临床病理特征的关系；通过 受试者工作特征曲线和危险分数分析法评估miR-4286 对胃癌的诊断效能；通过电化学发光免疫分析法检测 血清肿瘤标志物CEA、CA19-9 和CA72-4 的表达。结果 胃癌组患者血清中miR-4286 表达量高于对照祖 （P <0.05）。miR-4286 的表达水平与胃癌患者的临床分期、淋巴结转移及远处转移等无关（P >0.05），与肿 瘤细胞分化程度有关（P <0.05）。两组血清中CEA、CA19-9 表达量比较，差异无统计学意义（P >0.05），胃 癌组CA72-4 表达量较对照组升高（P <0.05）。miR-4286 诊断胃癌的曲线下面积为0.727，特异性和阳性预 测值分别为90% 和0.872，高于肿瘤标志物CA72-4。结论 miR-4286 在胃癌血清中高表达，其表达水平与 病理分化程度有关，可能成为胃癌临床诊断的潜在指标。

Abstract:

Objective To investigate the correlation between the expression level of serum miR-4286 and clinicopathological characteristics of gastric cancer, and analyze its efficacy and significance in clinical diagnosis of gastric cancer. Methods Real time fluorescence quantitative PCR was used to detect the expression of miR-4286 in serum samples of healthy control group and gastric cancer group. The correlation between the expression of miR-4286 and the clinicopathological features of gastric cancer was analyzed by statistical methods; Diagnostic efficacy of miR- 4286 for gastric cancer was assessed by receiver operating characteristic curve and risk score analysis; Expression of tumor markers CEA, CA19-9, and CA72-4 were detected by electrochemiluminescence immunoassay. Results The expression of miR-4286 in the serum of gastric cancer group was higher than that in healthy control group (Z =-4.644, P < 0.0001). The expression level of miR-4286 in gastric cancer group was not related to tumor clinical stage, lymph node metastasis and distant metastasis (P > 0.05), but it was negatively correlated with the degree of pathological differentiation of tumor cells (Z =-2.661, P < 0.01). Compared with the control group, there was no significant difference in the expression of CEA and CA19-9 in the serum of gastric cancer group (P > 0.05), but the expression of CA72-4 was increased (Z =-3.324, P < 0.01). The area under the curve of miR-4286 for diagnosing gastric cancer was 0.727, and its specificity and positive predictive value were 90% and 0.872, respectively, which was higher than that of tumor marker CA72-4. Conclusions MiR-4286 is highly expressed in gastric cancer serum, and its expression level is negatively correlated with pathological differentiation. It may be a potential diagnostic marker for gastric cancer.