神经病理性疼痛大鼠不同大小DRG神经元兴奋性分型的研究*

Type of excitability of DRG neurons in rats with neuropathic pain*

摘要:

目的 研究神经病理性疼痛状态下，不同大小背根神经节（DRG）神经元兴奋性分型的特点，探讨不同大小DRG神经元兴奋性分型与神经病理性疼痛的关系。方法 将SD大鼠随机分成正常组（Control组）、假手术组（Sham组）和坐骨神经分支选择性损伤模型组（SNI组）。运用热和冷刺激实验观察大鼠疼痛行为学变化；运用膜片钳技术记录不同大小DRG神经元兴奋性分型变化。结果 与Control组比较，SNI组大鼠热缩足潜伏期无改变，但冷刺激抬足时间增加（P?<0.05）；与Control组比较，SNI组大鼠DRG神经元的1型和2型细胞构成比增加，3型细胞构成比减少（P?<0.05）；在Control组内，与大细胞比较，中、小细胞3型细胞的构成比较低，而1型和2型细胞的构成比较高（P?<0.05）；在SNI组内，与大细胞比较，中细胞3型细胞构成比较低，1型和2型细胞构成比较高（P?<0.05），而小细胞与大细胞的构成比差异无统计学意义（P?>0.05）。结论 不同大小的DRG神经元兴奋性分型的改变是产生神经病理性疼痛的机制之一。

Abstract:

Objective To study the type of excitatory of DRG neurons in rats with neuropathic pain. Methods SD rats were randomly divided into normal group, sham operation group and SNI model group. The pain behavior changes of rats were observed by heat and cold stimulation experiments. The patch clamp technique was used to record the changes of excitability types of DRG neurons in different size. Results Compared with the normal group, the cold stimulation time was significantly increased (P??0.05). Conclusions The changes in excitatory typing of dorsal root ganglion cells with different sizes are potentially the mechanisms of neuropathic pain.