水通道蛋白3介导过氧化氢转运对宫颈癌HeLa细胞增殖、迁移的作用研究*

Inhibitory effect of aquaporin-3 on proliferation and migration of cervical carcinoma HeLa cells*

摘要:

目的 探讨水通道蛋白3（AQP3）介导肿瘤微环境中过氧化氢（H2O2）转运对宫颈癌HeLa细胞增殖、迁移的作用。方法 应用宫颈癌HeLa细胞，构建AQP3 shRNA慢病毒稳定转染宫颈癌细胞系，通过克隆形成、CCK-8、细胞平板克隆形成、Western blotting等比较H2O2处理前后细胞表型的差异。结果 成功构建AQP3-464- shRNA慢病毒稳定转染HeLa细胞系，AQP3 shRNA抑制肿瘤微环境中H2O2转运，抑制宫颈癌HeLa细胞增殖、迁移，AQP3 shRNA抑制肿瘤微环境中H2O2转运调控下游信号蛋白激酶B（PKB）。结论 AQP3介导宫颈癌HeLa细胞中H2O2跨膜转运，激发细胞内依赖第二信使H2O2的下游信号PKB，促进HeLa细胞的增殖、迁移。

Abstract:

Objective To investigate the role of aquaporin 3 (AQP3) in proliferation and migration of cervical cancer HeLa cells. Methods Construction of AQP3 shRNA overexpression in cervical cancer HeLa lines via lentivirus. CCK-8, cell clone formation and Western blotting were performed for phenotype conformation. Results AQP3-464-shRNA overexpression in HeLa cell line via lentivirus was successfully constructed. AQP3 shRNA inhibited hydrogen peroxide transportation in tumor microenvironment. Inhibition of proliferation and migration of cervical cancer HeLa cells was achieved through AQP3 shRNA. Treatment of AQP3 shRNA inhibited AKT activation. Conclusions AQP3 promotes the proliferation and migration of HeLa cells through transportation of H2O2 and downstream AKT signaling pathway.