Galectin-3、MMP-9和VEGF在子宫腺肌病组织中的表达及意义*

Clinical significance of expression of galectin-3, MMP-9 and VEGF in adenomyosis*

摘要:

目的 探讨半乳糖凝集素3（Galectin-3）、基质金属蛋白酶9（MMP-9）和血管内皮生长因子（VEGF）在子宫腺肌病在位内膜、异位内膜组织中的表达和意义。方法 采用逆转录聚合酶链反应（RT-PCR）和Western blotting分别检测Galectin-3、MMP-9和VEGF在20例子宫腺肌病在位内膜组织和20例正常对照内膜组织中mRNA和蛋白的相对表达。免疫组织化学法分别检测Galectin-3、MMP-9和VEGF在40例子宫腺肌病在位内膜、异位内膜组织和40例正常对照内膜组织中蛋白的表达，分析其在异位内膜组织中高表达的相关性。结果 与正常对照内膜比较，Galectin-3、MMP-9和VEGF在子宫腺肌病在位内膜组织中mRNA相对表达量升高（P?<0.05），蛋白相对表达量也升高（P?<0.05）。与正常对照内膜比较，Galectin-3和VEGF在子宫腺肌病内膜中高表达（P?<0.05）。Galectin-3和MMP-9（r?=0.493，P?=0.001）、Galecein-3和VEGF（r?=0.616，P?=0.000）、MMP-9和VEGF（r?=0.441，P?=0.004）高表达之间均呈正相关。结论 Galectin-3、VEGF和MMP-9在子宫腺肌病内膜组织中表达升高且呈正相关，可能共同参与子宫腺肌病的发生和异位侵袭。

Abstract:

Objective To investigate the expression and clinical significance of galectin-3, MMP-9 and VEGF in endometrial tissues of adenomyosis. Methods Relative expression of galectin-3, MMP-9 and VEGF in eutopic endometrial tissues of 20 cases of adenomyosis and 20 normal controls were detected by RT-PCR and Western blot. Immunohistochemical staining was performed to detect expression of galectin-3, MMP-9 and VEGF in eutopic endometrium and ectopic endometrium of 40 cases of adenomyosis as well as 40 cases of normal endometrium. The correlation of galectin-3, MMP-9 and VEGF in ectopic endometrium was analyzed. Results Levels of galectin-3, MMP-9 and VEGF demonstrated by RT-PCR and Western blot were increased significantly in eutopic endometrium compared with those in normal endometrium (P?