乳头状甲状腺癌中microRNA-599、BRD4表达及与临床病理特征和预后的关系

Clinical significance of microRNA-599 and BRD4 in papillary thyroid carcinoma

摘要:

目的 研究乳头状甲状腺癌（PTC）中microRNA-599（miR-599）、含溴结合域蛋白（BRD4）表达及与临床病理特征和预后的关系。方法 选取2005年5月—2007年3月云南省第一人民医院接受甲状腺手术的PTC患者52例。其中癌旁正常甲状腺组织41例，分离癌变组织和癌旁正常组织，分别检测组织中miR-599和BRD4的相对表达量，分析癌变组织中miR-599和BRD4表达与患者临床病理特征和预后的关系。结果 PTC患者癌变组织和癌旁正常组织中miR-599、BRD4的相对表达量差异有统计学意义（P?<0.05）；miR-599、BRD4相对表达量在不同TNM分期、肿瘤直径、肿瘤侵犯包膜、淋巴结转移患者中的差异有统计学意义（P?<0.05）；miR-599、BRD4高表达和低表达患者5及10年生存率差异有统计学意义（P?<0.05）；miR-599与BRD4的相对表达量呈负相关（r?=-0.872，P?=0.000）。结论 miR-599和BRD4与PTC发生、发展及预后密切相关，对临床诊断和治疗PTC及预测预后有重要意义。

关键词:甲状腺肿瘤;microRNA-599;含溴结合域蛋白/蛋白;病理学，临床;预后

Abstract:

Objective To study the expression and clinical significance of miR-599 and bromodomain-containing protein (BRD4) in papillary thyroid carcinoma (PTC). Methods A total of 52 PTC patients who received thyroid surgery in our hospital from May 2005 to March 2007 were selected. Totally 41 cases of para-cancer normal tissue as well as cancerous tissues were utilized for determination of expression of miR-599 and BRD4. Relationship between the expressions of miR-599 and BRD4 and clinicopathological features was analyzed. Results The relative expression of miR-599 and BRD4 in cancerous tissues was significantly different when compared with adjacent normal tissues (P?

Key words:thyroid neoplasms; microRNA-599; bromodomain-containing protein/protein; pathology, clinics; prognosis