蛴螬提取物对视网膜中央静脉阻塞模型兔小胶质 细胞CD68 表达的影响及机制研究

Effect and mechanism of grub extract on expression of CD68 in microglia of rabbits with central retinal vein occlusion

摘要:

目的 探讨蛴螬提取物对实验性兔视网膜静脉阻塞（RVO）的治疗作用及相关机制。方法 选 取40 只兔并随机分为空白组、模型组、复方血栓通组及蛴螬组。除空白组外，其余3 组采用光化学动力 法复制RVO 动物模型，于给药后7、14 及28 d 行荧光素眼底血管造影术（FFA），于给药后1、3、7、14 及28 d 行免疫组织化学检测白细胞分化抗原68（CD68）的表达。结果 FFA 检查显示，模型组给药7 d 后，中央静脉荧光渗漏、出血，出现无灌注区；复方血栓通组与蛴螬组出现无灌注区，但渗漏、出血较模型 组少；给药28 d 后，模型组渗漏、出血的静脉周围无灌注区面积增大，并出现新生血管；复方血栓通组渗 漏、出血明显减少；蛴螬组渗漏、出血基本吸收。复方血栓通组、蛴螬组与模型组给药后7、14 及28 d 的 无灌注区与视盘面积比值比较，差异有统计学意义（P <0.05）。免疫组织化学结果显示，各模型组CD68 表 达增加，复方血栓通和蛴螬提取物都使CD68 的阳性表达随时间的延长而减弱，但蛴螬组CD68 表达减弱 得更快。复方血栓通组、蛴螬组与模型组3、7、14 及28 d CD68 表达比较，差异有统计学意义（P <0.05）。 结论 复方血栓通与蛴螬提取物均不同程度抑制视网膜小胶质细胞的活化，而蛴螬提取物的抑制作用更明显， 说明其比单纯的活血化瘀药能更快地抑制小胶质细胞活化。

Abstract:

Objective To evaluate the therapeutic Effect and mechanism of grub extract on expression of CD68 in microglia of rabbits with central retinal vein occlusion (RVO). Methods Forty rabbits were randomly divided into 4 groups: blank group, model group, compound Xueshuantong group and grub group. Except for the blank group, the other three groups were used to establish RVO animal model by photochemical kinetic method. FFA examination was performed 7d, 14d, and 28d after administration, and immunohistochemical differentiation of antigen 68 (CD68) was stained 1d, 3d, 7d, 14d, and 28d after administration. The results were observed and analyzed. Results The results of FFA examination showed that there was non-perfusion area in the central venous fluorescence leakage and hemorrhage 7 days after administration in the model group. There was non-perfusion area in the compound Xueshuantong group and the grub group 7 days after administration, but the incidence of leakage and bleeding was less than that in the model group. On the 28th day, the area of the non-perfusion area around the vein leaking and bleeding in the model group increased, and new blood vessels appeared; the leakage and bleeding of the compound Xueshuantong group were significantly reduced; the leakage and bleeding of the grub group were basically absorbed. The ratio of non-perfusion area to optic disc area 7, 14 and 28 days after administration of compound Xueshuantong group, grub group and model group was significantly different (P < 0.05). The results of CD68 immunohistochemistry showed that the expression of CD68 was increased in all groups, and the positive expression of CD68 was attenuated with the increase of CD68, but the expression of CD68 was decreased more rapidly in the grub group. The expression of CD68 in compound Xueshuantong group, grub group and model group at 3, 7, 14 and 28 days was significantly different (P < 0.05). Conclusions Compound Xueshuantong and grub extract can inhibit the activation of retinal microglia in different degrees, and the inhibitory effect of grub extract was more obvious, indicating that grub extract can inhibit activation of microglia more quickly than simple blood stasis drug.