慢性根尖肉芽肿中TNF-α 与IL-34 表达的相关性

Correlation between IL-34 and TNF-α in the chronic periapical granulomas

摘要:

目的 探讨慢性根尖肉芽肿中肿瘤坏死因子α（TNF-α）和白细胞介素-34（IL-34）表达的 关系，并进一步研究TNF-α 在慢性根尖肉芽肿中的致病机制。方法 选取2018 年3 月—2018 年5 月就诊 于蚌埠医学院第二附属医院且临床及影像学诊断为慢性根尖肉芽肿的患者。9 例根尖肉芽肿组织作为实验组。 拔除智齿时切除的健康牙龈组织8 例作为对照组。应用免疫组织化学法检测两组TNF-α 和IL-34 蛋白的表 达，并应用图像处理软件分析两者的表达量及相关性。结果 实验组TNF-α、IL-34 相对表达量高于对照 组（P <0.05）；实验组TNF-α 与IL-34 呈正相关（r =0.896，P =0.001），对照组TNF-α 与IL-34 无相关性 （r =0.186，P =0.659）。结论 在慢性根尖肉芽肿中TNF-α 与IL-34 的表达有相关性。在慢性根尖肉芽肿中， TNF-α 可能通过IL-34 发挥致病作用。

Abstract:

Objective To study the correlation of TNF-α and IL-34 in the chronic apical granulomas and the pathogenic mechanism of TNF-alpha in chronic periapical granulomas. Methods Nine cases of chronic periapical granulomas were selceted as experimental samples. Eight cases of normal gingival tissues from the impacted third molars recommended for extraction were selected as normal control samples. The expressions of IL-34 protein and TNF-α protein in the experimental samples and control samples were detected by immunohistochemical analysis. The image J was used to analyze the expressions of IL-34 and TNF-α. Results The relative expression of TNF-a and IL- 34 in the experimental group was higher than that in the control group (P < 0.05); there was a positive correlation between TNF-a and IL-34 in the experimental group (r = 0.896, P = 0.001), but no correlation between TNF-a and IL-34 in the control group (r = 0.186, P = 0.659). Conclusions The relationship of TNF-a and IL-34 in the chronic periapical granulomas is demonstrated, and the TNF-α may play a pathogenic role through IL-34.