先天性肾积水患儿尿水通道蛋白-1、内皮素-1表达水平及临床意义

Clinical significance of urinary AQP-1 and ET-1 in children with congenital hydronephrosis

摘要:

目的 分析先天性肾积水患儿尿水通道蛋白（AQP-1）及内皮素-1（ET-1）的表达水平，并探索其临床意义。方法 选取40例先天性肾积水患儿作为实验组，并根据肾积水严重程度分为轻、中、重度积水。另选无泌尿系疾患儿童40例作为对照组。收集实验组术前晨尿、术中肾盂尿和术后第7天晨尿，以及对照组晨尿。测定实验组及对照组尿液AQP-1及ET-1表达水平，并进行尿AQP-1、ET-1水平的组间比较，及实验组术前、术中、术后尿AQP-1水平的组内比较。结果 轻度肾积水患儿尿AQP-1水平与对照组比较，差异无统计学意义（P?>0.05）；中度、重度肾积水患儿尿AQP-1水平则低于对照组（P?<0.05）；实验组患儿术中尿AQP-1水平低于术前水平（P?<0.05）；术后尿AQP-1水平高于术前水平（P?<0.05）；先天性肾积水患儿尿ET-1阳性率高于对照组（P?<0.05）。结论 尿AQP-1及ET-1水平可作为先天性肾积水患儿的辅助诊断指标。尿AQP-1水平还可作为肾积水患儿术后恢复的监测指标。

Abstract:

Objective To investigate clinical significance of urine quaporin-1 (AQP-1) and ET-1 (endothelin-1) in children with congenital hydronephrosis. Methods A total of 40 cases of children with congenital hydronephrosis and 40 healthy children were selected as observation group and control group, respectively. Patients were sub-grouped into mild, moderate and severe group based on severity of the disease. Pre-operative morning urine, intra-operative renal pelvis urine and morning urine at the 7th day after the operation were collected for the detection of ET-1, and AQP1. Results There was no significant difference in APQ-1 between mild group and control group (P?>?0.05). Obvious increase of APQ-1 was witnessed in moderate and severe group when compared with control group. In the observation group, intraoperative levels of AQP-1 was lowered while postoperative levels of AQP-1 was increased significantly compared with pre-operation (P?