醛糖还原酶抑制剂对屋尘螨诱导的人支气管 上皮细胞炎症因子的影响

Regulation of aldose reductase inhibitors on house dust mite (HDM) - induced bronchial epithelial inflammatory factors

摘要:

目的 测定醛糖还原酶抑制剂对屋尘螨诱导的人支气管上皮细胞NF-κB 及细胞因子表达水平 的影响，探索气道炎症的新干预措施。方法 将人支气管上皮细胞分为空白对照组（空白对照）、屋尘螨组（屋 尘螨）、屋尘螨+Zopol 组[ 屋尘螨+ 醛糖还原酶抑制剂唑泊司他（Zopol）] 及Zopol 组（醛糖还原酶抑制剂 Zopol），检测各组细胞NF-κB/p65 mRNA 的相对表达量、NF-κB/p65 核蛋白、上清液中IL-29 及IL-6 的蛋白含量。结果 屋尘螨+Zopol 组经屋尘螨及醛糖还原酶抑制剂共同作用24 h 后，人支气管上皮细胞的 NF-κB/p65 mRNA、NF-κB/p65 核蛋白相对表达量、IL-6 及IL-29 蛋白水平均低于屋尘螨组（P <0.05）， 但高于空白对照组（P <0.05），Zopol 组与空白对照组之间，差异无统计学意义（P >0.05）。结论 醛糖还原 酶抑制剂Zopol 调控了NF-κB 介导的炎症信号，减少人支气管上皮细胞炎症因子的表达。

Abstract:

Objective To explore new treatment for airway inflammation in the future, through the determination of the influence of aldose reductase inhibitors on expression of cytokine and NF-κB in human bronchial epithelial cells after the stimulus of house dust mites. Methods Human bronchial epithelial cells were divided into three groups: blank (group A), 300 ng/ml HDM (group B), 300 ng/ml HDM+aldose reductase inhibitors Zopo (group C) and aldose reductase inhibitors Zopol (group D). The relative expression of NF-kappa B/p65mRNA, NF-kappa B/ p65 nuclear protein and protein of IL-29 and IL-6 in cell supernatant were assayed. Results The data showed that after 24 hours of combined action of house dust mites and aldose reductase inhibitors, the NF-kappa B/p65 mRNA level, NF-kappa B/p65 nuclear protein level, IL-6 protein level and IL-29 protein level of human bronchial epithelial cells in group C were significantly lower than those of group B (P < 0.05), but higher than group A (P < 0.05). There were no significant differences between group D and group A (P > 0.05). Conclusions Aldose reductase inhibitor zopolrestat regulates inflammatory signaling mediated by NF-kappa B. The expression of inflammatory factor in human bronchial epithelial cells significantly reduces because of zopolrestat.