早期动脉粥样硬化患者的血清代谢组学研究

Serum metabolomics investigation of patients with early atherosclerosis

摘要:

目的 采用基于液相色谱- 质谱联用技术的代谢组学方法，探讨早期动脉粥样硬化（AS）患者 的血清生物标志物，为AS 的发生机制和早期诊断提供依据。方法 运用基于超高效液相色谱- 四极杆飞行 时间质谱技术对24 例健康志愿者（对照组）和27 例AS 患者（AS 组）的血清样本进行代谢组学分析。采用 正交偏最小二乘法判别分析方法对所得数据进行模式识别，分析两组代谢谱差异，筛选并鉴定两组差异代谢 产物。结果 血清样本中共鉴定分析6 个差异代谢产物，其中AS 患者血清中甜菜碱含量升高（P <0.05），而 磷脂酰胆碱等5 个代谢产物的水平降低（P <0.05）。结论 AS 发生早期，机体氨基酸代谢、脂肪酸代谢及三 羧酸循环等代谢通路发生改变，所鉴定代谢产物有望成为AS 早期筛查指标。

Abstract:

Objective To investigate the serum metabolic profiles of early atherosclerosis in patients by LCMS- based method to provide the basis for early diagnosis. Methods Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was applied to process the serum samples of 24 healthy volunteers and 27 atherosclerosis patients. Orthogonal to partial squares discriminant analysis was carried out for pattern recognition and characteristic metabolites identification between the two groups. Results There were 6 biomarkers identified after multivariate statistical analysis. The level of betaine was increased and 5 biomarkers such as phosphatidylcholine were decreased in the serum of atherosclerosis patients (P < 0.05). Conclusions Based on the analysis of metabolic pathways, amino acid metabolism, fatty acid metabolism and tricarboxylic acid cycle in atherosclerosis patients are disordered. These 6 different biomarkers can be helpful for the early diagnosing of atherosclerosis.