高原地区妊娠期高血压产妇胎盘Stat3、Survivin 及MMP-2 的表达水平及意义

Expression levels and significances of Stat3, Survivin and MMP-2 in placenta of pregnancy induced hypertension syndrome in plateau area

摘要:

目的 探究高原地区妊娠期高血压产妇胎盘中信号传导和转录活化因子3（Stat3）、Survivin 及基 质金属蛋白酶-2（MMP-2）的表达水平及意义。方法 选取青海红十字医院收治的30 例轻中度妊娠期高血压、 27 例重度妊娠期高血压及22 例正常者胎盘组织标本进行研究，用苏木精- 伊红染色观察各组胎盘组织变化， 逆转录聚合酶链反应、免疫组织化学法检测胎盘组织中Stat3、Survivin 及MMP-2 蛋白表达，Pearson 法分析 Stat3、Survivin 及MMP-2 与新生儿体重、胎盘的相关性。结果 轻中度组Stat3、Survivin、MMP-2 mRNA 水平和蛋白阳性率均较正常组下降（P <0.05），重度组均较轻中度组下降（P <0.05）。妊娠期高血压胎盘中 Stat3、Survivin、MMP-2 相互间及其与胎盘重量、新生儿体重呈正相关（P <0.05）。结论 胎盘中Stat3、 Survivin 及MMP-2 低表达可能与妊娠期高血压的发生及新生儿预后相关。

Abstract:

Objective To investigate the expression and significance of signal transduction and activator of transcription 3 (Stat3), Survivin, and matrix metalloproteinase-2 (MMP-2) in the placenta of pregnancy induced hypertension syndrome in plateau area. Methods A total of 30 cases placenta tissue specimens of patients with mild to moderate pregnancy induced hypertension, 27 cases of severe degree and 22 cases of normal puerperas were collected for study. HE staining was used to observe the changes of placenta tissues; the expressions of Stat3, Survivin, and MMP-2 proteins in placenta tissues were detected by RT-PCR and immunohistochemistry; the correlations among Stat3, Survivin, MMP-2 and neonatal weight, placenta were analyzed by Pearson correlation analysis. Results The levels of Stat3, Survivin, and MMP-2 mRNAs in three groups were significantly different (P < 0.05). Compared with the normal group, the expressions of Stat3, Survivin and MMP-2 mRNAs in placenta of mild to moderate pregnancy-induced hypertension group decreased (P < 0.05); the expressions of Stat3, Survivin and MMP-2 mRNAs in severe group were lower than those in mild and moderate group (P < 0.05). Stat3, Survivin, and MMP-2 were positively correlated with each other and they were positively correlated with placental weight and neonatal weight in pregnancy induced hypertension (P < 0.05). Conclusions The low expressions of Stat3, Survivin and MMP-2 in placenta may be related to the occurrence of pregnancy induced hypertension and the prognosis of the neonates.