小鼠经腰段鞘内置管的建立方法

Establishment of a novel intrathecal catheterization in mice lumber spinal subarachnoid space

摘要:

目的 介绍改良的小鼠经腰段鞘内置管的方法，旨在为神经学和疼痛学研究提供更安全、方便、高效的小鼠鞘内置管技术。方法 60只雄性昆明小鼠，随机分为改良组和Wu方法组，分别接受经改良和传统的Wu方法行小鼠鞘内置管术，记录两组小鼠导管泄漏率、瘫痪率、死亡率、置管成功率，以及热甩尾潜伏期（TFL）、50%机械缩足阈值（MWT）、导管滑脱率。解剖脊髓观察脊髓损伤情况及置管情况。结果 两组小鼠在死亡率、TFL、50% MWT方面比较，差异无统计学意义（P?>0.05），改良组与Wu方法组比较，成功率提高，瘫痪率、导管泄漏率、导管滑脱率降低（P?<0.05）。结论 改良的鞘内置管方法较传统的Wu方法更加安全易行。

Abstract:

Objective To introduce a modified method of catheterization in mice lumbar subarachnoid space with better safety, convenience and efficiency. Methods Male Kunming mice were randomly divided into two groups (n?=?30): the modified group and the Wu group. Mice received intrathecal catheterization. Catheter leakage rate, paralysis rate, mortality, and success rate, tail flick frequency, 50% mechanical withdrawal threshold, catheter slip rate, and spinal cord catheterization condition after dissecting spinal cord were recorded. Results There was no statistically significant difference in mortality, tail flick frequency, 50% mechanical withdrawal threshold between the two groups (P?>?0.05). The success rate was significantly improved while paralysis rate, the catheter leakage rate and the catheter slip rate were decreased obviously when compared with the Wu group (P?