载基因磁性脂质微泡的制备及对人HepG2细胞的生长抑制作用研究*

Effect of magnetic lipid microbubble-based gene vectors on human HepG2 cell growth*

摘要:

目的 探讨自制载基因磁性脂质微泡的制备方法及该微泡在联合磁感应热疗和基因治疗下对人HepG2细胞的生长抑制作用。方法 采用机械振荡法制备载基因磁性脂质微泡。对磁性脂质微泡进行表征分析及检测Fe3O4磁性脂质微泡的升温情况。利用pEGFP-C1观察磁性脂质微泡介导外源基因转染的效率。MTT法，流式细胞仪评价载基因磁性脂质微泡对人HepG2细胞生长情况的影响。结果 载基因磁性脂质微泡粒径小，性质稳定，具有良好的磁响应性能，可将外源WtP53基因转染至HepG2细胞系并高效表达，在磁场作用下具有升温恒温能力，在联合磁感应热疗和基因治疗下对人HepG2细胞有生长抑制作用。结论 载基因磁性脂质微泡为肿瘤的联合治疗开辟新的思路。

Abstract:

Objective To explore inhibitory effect of magnetic lipid microbubble based vectors on the growth of human HepG2 cells in vitro. Methods Magnetic lipid microbubbles based gene vectors were prepared by mechanical oscillation method. Fe3O4 magnetic nanoparticles and heating up of magnetic lipid microbubble Fe3O4 magnetic lipid microbubble were identified. PEGFP-C1 was utilized to observe the efficiency of foreign gene transfection. mediated bymagnetic lipid microbubbles. MTT method and flow cytometry were used to evaluate growth of HCC cells. Results The magnetic lipid microbubble contrast agent has a small particle size and stable properties, and has good magnetic response. The exogenous WtP53 gene can be transfected into HepG2 cell line and expressed constantly. Ability of self-heating up was achieved under the action of magnetic field. Gene therapy combination of magnetic induction hyperthermia and gene therapy in human HepG2 cells inhibited cellular growth. Conclusions Magnetic lipid microbubble based gene vectors may be a novel approach for cancer therapy.