高剂量D- 半乳糖复制C57BL/6J 衰老小鼠模型的研究

Aging model induced by high-dose D-galactose in C57BL/6J mice

摘要:

目的 探讨高剂量D- 半乳糖复制C57BL/6J 衰老小鼠模型的效果。方法 选用30 只9 周龄雄 性C57BL/6J 小鼠，随机分为3 组：500 mg/（kg·d）D- 半乳糖组、1 000 mg/（kg·d）D- 半乳糖组及对照 组，持续皮下注射8 周后解剖小鼠，测定血液和组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHPx） 及丙二醛（MDA）等，Western blotting 检测组织中血红素加氧酶-1（HO-1）的表达，并对部分组织行 苏木精- 伊红染色。结果 1 000 mg/（kg·d）D- 半乳糖组小鼠出现不同程度的脱毛现象。D- 半乳糖组小 鼠的SOD 和GSH-Px 低于对照组（P <0.05），而MDA 和HO-1 高于对照组（P <0.05）。D- 半乳糖组小鼠肝、 脑组织有不同程度的衰老相关病理学改变。结论 D- 半乳糖可以成功诱导小鼠衰老，是复制小鼠衰老模型 的较好选择。

Abstract:

Objective To evaluate the effect of the high-dose D-galactose inducing aging model in C57BL/6J mice. Methods Thirty C57BL/6J mice were divided into 3 groups: 500 mg/ (kg·d) D-galactose group, 1000 mg/ (kg·d) D-galactose group and the 0.9% saline group by daily subcutaneous injection for 8 weeks. The superoxide dismutase, glutathione peroxidase and malondialdehyde levels in serum, liver and brain were evaluated. The expression of heme oxygenase-1 expression in liver and brain was detected by western blotting. Tissues were observed by H&E staining. Results There were no differences in the appearance between 500 mg/ (kg·d) group and control group, but the mice in 1000 mg/ (kg·d) group showed the phenomenon of hair loss. The activities of superoxide dismutase and glutathione peroxidase significant decreased in the groups treated with D-galactose compared with control group (P < 0.05), and the concentration of the malondialdehyde was significantly higher than the control group (P < 0.05). The expression of heme oxygenase-1 was increased in the D-galactose group. There were Pathological changes in D-galactose groups. Conclusions The D-galactose could induce the aging model and the dose of 1000 mg/ (kg·d) D-gal could cause the appearance change in C57BL/6J mice.