离心对不同层次脂肪移植成活率的影响*

Effect of centrifugation fraction on survival rate of lipoaspirate*

摘要:

目的 探究离心后不同层次脂肪的移植成活率，以便更好地指导临床应用。方法 选取新西兰兔腹股沟脂肪，按照Coleman方法离心脂肪，并将其分为上、中、下3层，每层各1 ml移植于背部皮下；3个月后，对移植物进行组织学观察、测量体积及CD31免疫组织化学染色。结果 下层脂肪移植物成活体积，血管密度及成活质量均高于上层和中层脂肪移植物（P?<0.05），而中层和上层脂肪移植物成活体积、血管密度脂肪成活质量比较，差异无统计学意义（P?>0.05）。结论 按Coleman方法离心后的下层的脂肪成活率及成活质量较好，可以作为临床脂肪移植的理想选择。

Abstract:

Objective To explore the effect of centrifugation fraction on survival rate of lipoaspirate. Methods The inguinal fat tissue of New Zealand rabbits was harvested and centrifuged by Coleman procedure. Centrifuged lipoaspirate was divided into upper, middle and lower layers which were transplanted into the back of rabbits. Three months later, the fat grafts were assessed by survival volume, histological characteristics and immunohistochemical staining for CD31. Results The volume retention, density of vessel and survival quality of lower layers fat grafts were significantly higher than those of the other two layers (P??0.05). Conclusions The lower centrifuged layer may be an ideal candidate for fat grafts.