小檗碱对D- 半乳糖诱导多囊卵巢综合征 模型小鼠的影响

Effect of berberine on D-galactose-induced polycystic ovary syndrome in mice

摘要:

目的 探讨小檗碱对D- 半乳糖诱导的小鼠多囊卵巢综合征（PCOS）模型的影响。方法 20 周 龄ICR 小鼠根据Poretsky 模型复制方法腹腔注射D- 半乳糖，连续21 d 复制PCOS 模型，小檗碱灌胃治疗 21 d。监测小鼠饮食和体重变化；取血测定小鼠空腹血糖；观察小鼠动情周期及排卵情况；取卵巢组织观 察小鼠卵巢超微结构及形态变化。结果 小檗碱组与D- 半乳糖组进食量、血糖值比较，差异无统计学意义 （P >0.05），但体重降低（P <0.05）。小檗碱可改善D- 半乳糖诱导的PCOS 模型小鼠动情周期紊乱，改善卵 巢组织结构。黄体细胞内脂滴数量减少，颗粒细胞质内细胞器完整，卵泡膜细胞内脂滴及粗面和滑面内质网 数量减少，并可见少量凋亡的卵泡膜细胞。结论 小檗碱对D- 半乳糖诱导的小鼠PCOS 模型具有治疗作用。

Abstract:

Objective To explore the effect of berberine on d-galactose-induced polycystic ovary syndrome (PCOS) in mice. Methods 20-week-old ICR mice were injected with d-galactose intraperitoneally according to the Poretsky modeling method, then PCOS model was constructed for 21 consecutive days and berberine was administered for 21 days. The changes in diet and weight of mice were monitored; blood glucose was measured; estrus cycle and ovulation were monitored; ovarian tissue was taken to observe the ultrastructure and morphological changes. Results Compared with d-galactose model group, berberine group had no significant difference in food intake and blood glucose (P > 0.05), but significantly reduced body weight (P < 0.05). Berberine can ameliorate the estrous cycle disorder and ovarian tissue structure in d-galactose induced polycystic ovary syndrome model mice. The number of lipid drops in luteal cells decreased, the cytoplasmic organelles in granulosa cells were intact, the number of lipid drops in follicular membrane cells and rough and smooth endoplasmic reticulum were significantly reduced, and a small number of apoptotic follicular membrane cells were observed. Conclusions Berberine has therapeutic effect on d-galactose-induced polycystic ovary syndrome in mice.