胆汁及胆囊黏膜中骨桥蛋白、瘦素、内脂素 及脂联素的表达及意义

Expression and significance of osteopontin, leptin, visfatin and adiponectin in bile and gallbladder mucosa

摘要:

目的 通过检测胆固醇结石患者胆汁成分和胆囊黏膜基因的表达以探索胆固醇结石的成因。 方法 采用酶联免疫吸附总试验、氧化酶法和咖啡因法检测胆固醇结石患者胆汁中胆固醇、瘦素、内脂素、 脂联素、磷脂、总胆汁酸、胆固醇饱和指数（CSI）水平；免疫组织化学、Western blotting 检测胆囊黏膜组 织中骨桥蛋白（OPN）的表达。结果 观察组患者胆汁CSI、胆固醇、胆固醇百分比、总脂、瘦素及内脂素 浓度较对照组升高（P <0.05），总胆汁酸、磷脂和脂联素较对照组下降（P <0.05）；观察组胆囊黏膜中OPN 的平均光密度值和蛋白相对表达量较对照组高（P <0.05）。结论 OPN、内脂素、脂联素可能是胆固醇结石 形成的共同因素。

Abstract:

Objective To explore the cause of cholesterol gallstones through detecting the composition of bile and expression of genes of gallbladder mucosa in patients with cholesterol gallstone. Methods We analyzed the composition of bile including leptin, visfatin, adiponectin, phospholipid, bile acid, cholesterol and cholesterol saturation index (CSI) in patients with cholesterol gallstone by ELISA method, oxidase-peroxidase method and caffeine method, and the expression of osteopontin in gallbladder mucosa was detected by immunohistochemistry and Western blot assay. Results The CSI, percentage of cholesterol and concentration of cholesterol, total lipid, leptin and visfatin in gallstone group were significantly higher than those in the control group (P < 0.05); concentration of phospholipid, bile acid and ADPN were lower than those in control group (P < 0.05); expression of OPN for gallbladder mucosa in gallstone group were significantly enhanced (P < 0.05). Conclusions OPN, Leptin, Visfatin and ADPN may be the common factors of cholesterol stone formation.