HBV相关性肝病患者外周血PBMCs表面Tim-3、PD-1表达水平检测及意义

Detection and significance of Tim-3 and PD-1 expression levels on the surface of PBMCs in peripheral blood of patients with HBV-related liver diseases

摘要:

目的 分析乙型肝炎病毒（HBV）感染后相关肝病患者外周血单个核细胞（PBMCs）表面T细胞免疫球蛋白黏蛋白分子-3（Tim-3）、程序性死亡受体-1（PD-1）表达水平变化及意义。方法 选取2017年 1～12月川北医学院附属医院HBV携带者20例，慢性乙型肝炎（CHB）患者30例，重型乙型肝炎患者20例，乙肝肝硬化患者30例，肝细胞癌患者20例，以同期20例健康体检者作为健康对照组。检测各研究对象外周血PBMCs表面Tim-3、PD-1表达水平。结果 PBMCs表面Tim-3、PD-1表达水平在健康对照组最低，与HBV携带组比较差异无统计学意义（P?>0.05），随着病情加重，PBMCs表面Tim-3、PD-1表达水平逐渐升高，在重型乙型肝炎组、肝细胞癌组最高，各组患者PBMCs表面Tim-3、PD-1表达水平与健康对照组、HBV携带者组比较，差异有统计学意义（P?<0.05）；HBV感染患者PBMCs表面Tim-3、PD-1表达水平与HBV DNA载量呈负相关（r?=-0.431和-0.422，均P?<0.05），与ALT、AST水平呈正相关（r?=0.214、0.325、0.234和0.354，均P?<0.05）；HBV感染患者总体PBMCs表面Tim-3的表达水平与PD-1表达量呈正相关（r?=0.967，P?<0.05）。结论 免疫负性调节因子Tim-3、PD-1与HBV相关性肝病患者肝组织炎症及纤维化发生相关，对Tim-3、PD-1水平调节可能为其临床免疫治疗提供新思路。

Abstract:

Objective To analyze change and meaning of T cell immune globulin sticky protein molecules -3 (T cell immunoglobulin mucin molecule-3, Tim-3) and procedural death receptor-1 (programmed cell death protein-1, PD-1) on the surface of peripheral blood mononuclear cells (Human peripheral blood mononuclear cells, PBMCs) in patients with hepatitis B virus (hepatitis B virus, HBV) related liver disease. Methods From January 2017 to December 2017, 20 hepatitis B virus carriers, 30 chronic hepatitis B patients, 20 severe hepatitis B patients, 30 hepatitis B cirrhosis patients and 20 hepatitis B liver cancer patients were compared with 20 healthy subjects selected as the healthy control group, and the levels of Tim-3 and PD-1 on the surface of PBMCs in the peripheral blood of all subjects were tested. Results Levels of Tim-3 and PD-1 on PBMCs surface were the lowest in healthy control group, compared with those in hepatitis B virus carriers group, and the difference had no statistical significance (P?>?0.05). As the aggravating of illness, the levels of Tim - 3 and PD-1 on PBMCs surface increased, which was the highest in severe hepatitis B group and hepatitis B liver cancer group. The difference among the levels of Tim- 3 and PD-1 on PBMCs surface in other groups were statistically significant, compared with those in healthy group and hepatitis B virus carrier group (P?