siRNA沉默肿瘤坏死因子α诱导蛋白2表达对胶质瘤细胞侵袭及增殖的影响*

Effect of siRNA silencing TNFAIP2 expression on invasion and proliferation of glioma cells*

摘要:

目的 探讨沉默肿瘤坏死因子α诱导蛋白2（TNFAIP2）表达对胶质瘤细胞侵袭及增殖的影响。方法 使用Lipofectamine 2000介导siRNA转染胶质瘤细胞U87和U251下调TNFAIP2 mRNA的表达；运用细胞划痕及Transwell实验检测沉默TNFAIP2表达对U87及U251细胞迁移、侵袭的影响；通过CCK-8实验检测沉默TNFAIP2表达对胶质瘤细胞U87及U251增殖的影响。结果 Lipofectamine 2000介导si-TNFAIP2转染U87及U251细胞可下调TNFAIP2 mRNA的表达；沉默TNFAIP2表达可降低胶质瘤细胞U87和U251迁移侵袭的能力；沉默TNFAIP2表达可抑制U87及U251细胞的增殖。结论 siRNA沉默TNFAIP2表达可抑制胶质瘤细胞的侵袭和增殖。

Abstract:

Objective To investigate the effect of silencing TNFAIP2 (Tumor Necrosis Factor Alpha Induced Protein 2) expression on the invasion and proliferation ability of glioma cells. Methods Lipofectamine 2000 mediated siRNA transfecting U87 and U251 cells was used to silence the expression of TNFAIP2; and the effect of silencing TNFAIP2 expression on the migration, invasion and proliferation of U87 and U251 cells were detected by cell scratch and Transwell assay; the effect of silencing TNFAIP2 expression on proliferation of U87 and U251 glioma cells were measured by CCK-8 cell proliferation assay. Results U87 and U251 cells transfected by Lipofectamine 2000 mediated si-TNFAIP2 could significantly down-regulate the expression of TNFAIP2; Silencing TNFAIP2 expression significantly reduced the ability of migration and invasion of glioma cells U87 and U251; silencing the expression of TNFAIP2 could significantly inhibit the invasion and proliferation of U87 and U251 cells. Conclusions Silencing TNFAIP2 expression by SiRNA can effectively inhibit the invasion and proliferation of glioma cells.