水通道蛋白1和肿瘤坏死因子受体相关蛋白1在人脑胶质瘤中的表达及相关性*

Expression and correlation of AQP1 and TRAP1 in human glioma*

摘要:

目的 研究水通道蛋白1（AQP1）和肿瘤坏死因子受体相关蛋白1（TRAP1）在脑胶质瘤中的表达及其之间的相关性。方法 选取66例人脑胶质瘤和15例正常脑组织标本，经Western blotting及逆转录聚合酶反应（RT-PCR）对其AQP1、TRAP1 mRNA及蛋白表达进行检测分析。结果 ①在人脑胶质瘤中AQP1、TRAP1 mRNA及蛋白表达水平较对照组上调（P?<0.05）；②在高级别胶质瘤（Ⅲ、Ⅳ级）中AQP1、TRAP1 mRNA及蛋白表达与低级别胶质瘤（Ⅰ、Ⅱ级）比较，前者表达水平上调（P?<0.05）；③AQP1、TRAP1 mRNA及蛋白在脑胶质瘤的表达无相关性（rs?=0.074和0.058，P?=0.065和0.080）。结论 AQP1和TRAP1表达与脑胶质瘤的肿瘤分期有关，恶性程度越高其表达量也越高，但两者与脑胶质瘤的病理进展无协同性。

Abstract:

Objective To investigate the correlation and expression of aquaporin 1 (AQP1) and tumor necrosis factor receptor-associated protein 1 (TRAP1) in human gliomas. Methods The expression of AQP1 and TRAP1 mRNA and protein were detected by Western blotting and reverse transcription-polymerase reaction (RT-PCR) in 66 human glioma specimens and 15 normal brain specimens. Results ① Compared with the control group, the mRNA and protein expression of AQP1 and TRAP1 in human gliomas were increased (P?