肺癌食管癌缺失基因1及其甲基化在鼻内翻性乳头状瘤中的表达研究*

Research of expression of DLEC1 and its methylation in patients with nasal inverted papilloma*

摘要:

目的 探讨肺癌食管癌缺失基因1（DLEC1）表达及其甲基化在鼻内翻性乳头状瘤（NIP）中表达的临床意义及与Ki-67表达的关系。方法 收集30例NIP组织作为NIP组，20例鼻黏膜组织作为对照组，采用甲基化特异性聚合酶链反应（MSP）检测DLEC1基因启动子区域甲基化情况，免疫组织化学法检测DLEC1和Ki-67的表达。结果 30例NIP组织中DLEC1基因启动子甲基化比例为43.3%，高于正常组织（2.0%）（P?<0.05）；NIP组DLEC1失表达率为76.7%，高于对照组45.0%（P?<0.05）；NIP组Ki-67阳性表达率为60.0%，高于对照组10.0%阳性表达率（P?<0.05）；NIP组织中DLEC1的表达与Ki-67的表达呈负相关（P?<0.05）。结论 DLEC1基因启动子甲基化在NIP患者中表达较高，DLEC1在NIP中表达下调且与Ki-67呈负相关，DLEC1表达缺失可能与NIP的发病相关。

Abstract:

Objective To investigate the clinical value of DLEC1 gene expression and its methylation in nasal inverted papilloma (NIP) and its relationship with Ki-67 expression. Methods Thirty NSCLC tissues were collected as NIP group and 20 nasal mucosa tissues as control group. Methylation specific polymerase chain reaction (MSP) was used to detect the methylation of the DLEC1 gene promoter region. The expression of DLEC1 and Ki-67 was detected by immunohistochemistry. Results The methylation ratio of DLEC1 gene promoter in NIP tissues was 43.3%, which was higher than that in normal tissue (2.0%) (P?