TGF-β 通过Lnc-ATB 促进食管癌细胞 侵袭转移的机制研究

Mechanism of TGF-β promoting invasion and metastasis of esophageal cancer cells through Lnc-ATB

摘要:

目的 探讨转化生长因子-β（TGF-β）对食管癌细胞侵袭转移的影响及其作用机制。 方法 将食管癌细胞加入TGF-β 后，通过实时荧光定量聚合酶链反应检测食管癌细胞EC9706 中被TGF-β 活化的长链非编码RNA（Lnc-ATB）的表达情况，通过Transwell 实验检测TGF-β 对EC9706 细胞侵袭转移的 影响；沉默EC9706 细胞中Lnc-ATB 的表达，给予或不给予TGF-β 刺激，利用Transwell 实验检测EC9706 细 胞侵袭转移的情况。结果 Lnc-ATB-siRNA 组Lnc-ATB 较Lnc-ATB-NC 组低（P <0.05），Lnc-ATB siRNA 可有效沉默EC9706 细胞中Lnc-ATB 的表达。TGF-β 组TGF-β 处理后EC9706 细胞的侵袭和转移细胞数均 高于对照组（P <0.05）。TGF-β+Lnc-ATB-NC 组TGF-β 通过Lnc-ATB 上调EC9706 细胞侵袭及转移能力 高于其他组（P <0.05）；空白组细胞侵袭能力高于TGF-β+Lnc-ATB-siRNA 组和Lnc-ATB-siRNA 组（P <0.05）。 结论 食管癌中TGF-β 通过上调Lnc-ATB 的表达，促进细胞的侵袭及转移。

Abstract:

Objective To investigate the function and mechanism on invasion and metastasis of TGF-β in esophageal carcinoma. Methods EC9706 cells were treated with TGF-β, and then Lnc-ATB expression in EC9706 cells was detected by quantitative real time PCR (qRT-PCR); the invasion and metastasis of EC9706 cells were analyzed by Transwell; after silencing the expression of Lnc-ATB in EC9706 cells, treated with TGF-β or not, the invasion and metastasis of EC9706 cells was analyzed by Transwell. Results The expression of Lnc-ATB was higher in Lnc-ATB-siRNA group compared with Lnc-ATB-NC group (P < 0.05), and Lnc-ATB siRNA can effectively silence the expression of Lnc-ATB in EC9706 cells; the invasion and metastasis of EC9706 cells was significantly upregulated after treated with TGF-β (P < 0.05); the ability of invasion and metastasis promoted by Lnc-ATB after treated with TGF-β of EC9706 cells in TGF-β+Lnc-ATB-NC group was higher than in other groups (P < 0.05); the ability of invasion in control group was higher than in TGF-β+Lnc- ATB-siRNA group and Lnc-ATB-siRNA group (P < 0.05). Conclusions TGF-β promote the invasion and metastasis of cells via up-regulating the expression of Lnc-ATB in esophagus cancer.