胃癌组织中microRNA-144-3p 水平 及与临床病理特点和预后的关系

Relationship of miR-144-3p level with clinical pathological features and prognosis in gastric cancer

摘要:

目的 探讨胃癌组织中microRNA-144-3p（miR-144-3p）水平，以及与临床病理特点和预后 的关系。方法 选取2011 年1 月—2013 年12 月在义乌市中心医院进行手术切除的100 例胃癌患者胃癌组织 及其癌旁组织标本。采用实时荧光定量聚合酶链反应测定胃癌和癌旁组织中miR-144-3p 水平。结果 胃癌 组织中 miR-144-3p 低于癌旁组织（0.53±0.12） VS（ 1.00±0.11）（P <0.05）。不同临床分期、淋巴结转移及 分化程度胃癌患者miR-144-3p 水平比较，差异有统计学意义（P <0.05）；不同年龄和性别胃癌患者miR- 144-3p 水平比较，差异均无统计学意义（P >0.05）。miR-144-3p 高表达者5 年总生存率和无病生存率高于 低表达者（P <0.05）。Cox 多因素分析结果显示，miR-144-3p 低表达[Rl ^ R=3.627（95% CI ：1.514，8.783）]、 临床分期[Rl ^ R=1.627（95% CI ：1.073，4.735）]、淋巴结转移[Rl ^ R=4.025（95% CI ：1.043，8.931）] 及分化程 度[Rl ^ R=2.042（95% CI ：1.098，6.427）] 为影响胃癌预后的危险因素。结论 胃癌组织中miR-144-3p 水平 降低，其与临床分期、淋巴结转移、分化程度及预后关系密切。

Abstract:

Objective To investigate the relationship of miR-144-3p level with clinical pathological features and prognosis in gastric cancer. Methods The specimens of gastric cancer tissues and adjacent tissues from 100 patients with gastric cancer in Yiwu Central Hospital from January 2011 to December 2013 were selected for this study. The level of miR-144-3p in gastric cancer and paracancerous tissues were determined by real-time fluorescent quantitative polymerase chain reaction. Results The level of miR-144-3p in gastric cancer tissues (0.53±0.12) was lower than that in adjacent tissues (1.00±0.11) (P < 0.05). There were significant differences in miR-144-3p levels of gastric cancer tissues with different TNM stages, lymph node metastasis and differentiation (P < 0.05). There were no significant difference in miR-144-3p levels of gastric cancer tissues with different ages and genders (P > 0.05). The 5-year cumulative survival rate and disease-free survival rate of patients with high miR-144-3p expression were higher than those with low miR-144-3p expression (P < 0.05). The results of Cox multivariate analysis showed that miR-144-3p low expression [Rl ^ R=3.627, (95% CI: 1.514, 8.783)], TNM stage [Rl ^ R=1.627, (95% CI: 1.073, 4.735)], lymph node metastasis [Rl ^ R=4.025, (95% CI: 1.043, 8.931)] and differentiation [Rl ^ R=2.042, (95% CI: 1.098, 6.427)] were independent risk factors for the prognosis of gastric cancer (P < 0.05). Conclusions The level of miR-144-3p is decreased in gastric cancer tissues. The level of miR-144-3p is closely related to the diameter, depth of invasion,TNM stage, lymph node metastasis, differentiation degree and prognosis of gastric cancer.