大肠癌中自噬相关蛋白与JAK2/STAT3 信号通路的相关性

Relationship between autophagy relative proteins and JAK2/ STAT3 signaling pathway in colorectal carcinoma

摘要:

目的 探讨大肠癌发生、发展过程中自噬相关蛋白LC3、p62 与JAK2/STAT3 信号通路的关系。 方法 采用免疫组织化学法检测93 例大肠癌组织和癌旁组织中LC3、p62、p-JAK2 和p-STAT3 的蛋白表 达，并对其阳性表达率进行比较分析。结果 大肠癌中LC3、p-JAK2 和p-STAT3 阳性表达率高于癌旁组织 （P <0.05），高中分化大肠癌LC3、p-JAK2 和p-STAT3 蛋白阳性表达率低于低分化大肠癌（P <0.05），有淋 巴结转移患者LC3、p-JAK2 和p-STAT3 蛋白阳性表达率高于无淋巴结转移（P <0.05）。大肠癌p62 蛋白阳 性表达率低于癌旁组织（P <0.05），高中分化大肠癌p62 蛋白阳性表达率高于低分化大肠癌（P <0.05），有淋 巴结转移患者p62 蛋白阳性表达率低于无淋巴结转移（P <0.05）。LC3 蛋白表达与p-JAK2、p-STAT3 蛋白 表达均呈正相关（r s=0.315 和0.295，P <0.05），而p62 蛋白与p-JAK2、p-STAT3 蛋白表达均呈负相关（r s=-0.320 和-0.326，P <0.05）。结论 LC3、p62 在大肠癌中表达增加可能与JAK2/STAT3 信号通路有关，为大肠癌的 发病机制研究提供可能的理论基础。

Abstract:

Objective To explore the relationship of autophagy relative proteins LC3 and p62 with JAK2/ STAT3 signaling pathway in colorectal carcinoma (CRC). Methods The expressions of LC3, p62, p-JAK2, p-STAT3 in the CRC and adjacent tissues were detected by immunohistochemistry. Results The positive rates of LC3, p-JAK2 and p-STAT3 in colorectal cancer were higher than those in adjacent tissues (P < 0.05), and the positive rates of LC3, p-JAK2 and p-STAT3 in high and middle differentiated colorectal cancer were lower than those in low differentiated colorectal cancer (P < 0.05). The positive rates of LC3, p-JAK2 and p-STAT3 in patients with lymph node metastasis were higher than those in patients without lymph node metastasis (P < 0.05). The positive expression rate of p62 protein in colorectal cancer was lower than that in adjacent tissues (P < 0.05), and that in high differentiated colorectal cancer was higher than that in low differentiated colorectal cancer (P < 0.05). The positive expression rate of p62 protein in patients with lymph node metastasis was lower than that in patients without lymph node metastasis (P < 0.05). LC3 protein was positively correlated with p-JAK2 and p-STAT3 protein expression (rs = 0.315 and 0.295, P < 0.05), while p62 protein was negatively correlated with p-JAK2 and p-STAT3 protein expression (rs = -0.320 and-0.326, P < 0.05). Conclusions The expressions of LC3 and p62 were increased in CRC, and may be related to the JAK2/STAT3 signaling pathway, which may contribute to the pathogenesis of CRC.