rhGLP-1（7-36）联合他克莫司对人肝细胞Akt的作用研究*

Effect of rhGLP-1 (7-36) combined with FK506 on expression of Akt in human hepatocytes*

摘要:

目的 观察重组人胰高血糖素样肽-1（7-36）[rhGLP-1（7-36）]联合他克莫司作用于人肝细胞系HL7702细胞系后对Akt表达的影响。方法 选用处于对数生长期的人肝细胞系HL7702，分为4组：对照组、G组、F组及GF组。对照组用等量的培养基处理90?min；G组用含100?nmol/L的rhGLP-1（7-36）培养基处理90 min； F组用含5?mg/L的他克莫司处理90?min；GF组用含100?nmol/L的rhGLP-1（7-36）及5?mg/L的他克莫司处理90?min。Western blotting检测各组Akt蛋白表达水平。结果 各组p-AktThr308相对表达水平差异有统计学意义（P?< 0.05）；G组、F组及GF组p-AktThr308相对表达水平与对照组比较，差异有统计学意义（P?<0.05），G组、F组及GF组均升高；各组p-AktSer473相对表达水平差异无统计学意义（P?>0.05）；G组、F组及GF组p-AktSer473相对表达水平与对照组比较，差异无统计学意义（P?>0.05）。结论 rGLP-1（7-36）联合他克莫司能使Akt蛋白活化，提示GLP-1可能不依赖Akt蛋白改善他克莫司引起的肝脏脂质代谢紊乱。

Abstract:

Objective To investigate the effect of rhGLP-1 (7-36) combined with FK506 on the expression of Akt in human liver HL7702 cells. Methods Human liver HL-7702 cell line in logarithmic phase were divided into 4 groups: control group, group G, group F, group GF. The control group was treated with the same amount of medium as the experiment groups for 90 minutes. Group G was treated with 100 nmol/L rhGLP-1 (7-36) medium for 90 minutes. Group F was treated with 5?mg/L FK506 medium for 90 minutes. Group GF was treated with 100?nmol/L rhGLP-1 (7-36) and 5 mg/L FK506 medium for 90 minutes. We used western blotting to detect the expression of Akt protein in each group. Results There was significant difference in the expression of p-AktThr308 among all the groups (P??0.05). Conclusions Akt protein can be activated in human liver HL7702 cells with rhGLP-1 (7-36) combined with FK506, which means GLP-1 may not depend on Akt protein to improve hepatic lipid disorder induced by FK506.