结直肠癌患者外周血和癌组织中Vδ1 T和Vδ2 T细胞研究

Analysis of Vδ1 T and Vδ2 T cell in colorectal cancer patients from peripheral blood and tumor tissues

摘要:

目的 观察结直肠癌患者外周血和癌组织中Vδ1 T和Vδ2 T细胞比例和功能的变化，探讨其在结直肠癌发生、发展中的作用。方法 收集衢州市人民医院20例健康体检者（对照组）外周血，以及20例结直肠癌患者（癌症组）外周血和癌组织，通过流式细胞术检测对照组外周血，以及癌症组外周血、癌旁组织和癌组织Vδ1 T 和Vδ2 T细胞的比例；通过抗体扩增能力检测对照组外周血，以及癌症组外周血、癌旁组织和癌组织Vδ1 T和Vδ2 T细胞的增殖能力；通过增殖实验检测对照组外周血，以及癌症组外周血、癌旁组织和癌组织Vδ1 T细胞的免疫抑制能力。结果 对照组外周血Vδ1 T细胞比例为（1.1±0.5）%，癌症组外周血Vδ1 T细胞比例为（3.2±0.9）%， 两者比较，差异有统计学意义（P?<0.05），癌症组外周血Vδ1 T细胞比例高于对照组；对照组外周血Vδ2 T细胞比例为（4.5±1.5）%，癌症组外周血Vδ2 T细胞比例为（2.2±0.7）%，两者比较，差异有统计学意义（P?<0.05），癌症组外周血Vδ2 T细胞比例低于对照组。癌症组癌旁组织γδ T细胞经抗体扩增后，总γδ T细胞比例为（85.3± 18.3）%，Vδ1 T细胞比例为（24.5±12.8）%，Vδ2 T细胞比例为（55.3±15.2）%；癌症组癌组织γδ T细胞经抗体扩增后，总γδ T细胞比例为（84.9±12.6）%，Vδ1 T细胞比例为（60.6±13.7）%，Vδ2 T细胞比例为（21.8±12.6）%。癌症组外周血和癌组织Vδ1 T细胞的免疫抑制功能增强（P?<0.05），癌旁组织Vδ1 T细胞的免疫抑制功能亦增强（P?<0.05），但仍低于癌症组外周血和癌组织的Vδ1 T细胞免疫抑制能力。结论 癌症组外周血和肿瘤组织中Vδ1 和Vδ2 T细胞比例失衡和功能的改变可能与肿瘤逃逸相关。

关键词:结直肠肿瘤;T细胞;γδ T细胞;免疫抑制;增殖

Abstract:

Objective To observe the changes of the proportion and function of Vδ1 T and Vδ2 T cells in peripheral blood and tumor tissues of patients with colorectal cancer, and to discuss its role in colorectal cancer development. Methods Totally 20 cases of healthy control peripheral blood, 20 cases of peripheral blood and cancer tissues of colorectal cancer patients were collected. The proportions of Vδ1 T and Vδ2 T cells in peripheral blood of health control, peripheral blood of colorectal cancer patients, infiltration of cancer-adjacent tissues and cancer tissue were analyzed by flow cytometry. The proliferation ability of Vδ1 T and Vδ2 T cells in peripheral blood of health control, peripheral blood of colorectal cancer patients, infiltration of cancer-adjacent tissues and cancer tissue were detected by antibody amplification. The inhibit effect of Vδ1 T cells in peripheral blood of health control, peripheral blood of colorectal cancer patients, infiltration of cancer-adjacent tissues and cancer tissue were analyzed by proliferation experiment. Results The proportion of Vδ1 T cells in peripheral blood of healthy controls was (1.1?±?0.5) %, and the proportion of Vδ1 T cells in peripheral blood of patients with colorectal cancer was (3.2?±?0.9) %, and the difference was statistically significant (P?

Key words:colorectal neoplasms; t-lymphocytes; γδ t cells; immunosuppression; cell proliferation