节律基因clif单核苷酸多态性与老年急性心肌梗死患者的相关性研究

Association of circadian gene clif single nucleotide polymorphisms with acute myocardial infarction in elderly patients

摘要:

目的 探讨节律基因clif单核苷酸多态性与老年急性心肌梗死（AMI）患者的相关性。方法 回顾性分析2016年7月—2018年3月永康市第一人民医院收治的、符合纳入标准的老年AMI患者126例作为病例组，同期选择健康体检者132例作为对照组。采用单碱基引物延伸法基因分型检测rs2583913及rs1071592的基因型和等位基因；观察慢病毒干扰沉默clif基因小鼠的血浆凝血酶调节蛋白（TM）及纤溶酶原激活抑制物-1（PAI-1）mRNA及其蛋白表达水平。结果 病例组与对照组中rs2583913基因型及等位基因频率分布情况比较，差异均无统计学意义（P?>0.05）；两组rs1071592基因型及等位基因频率分布情况比较，差异均有统计学意义（P?<0.05）；慢病毒转染组clif、PAI-1及TM mRNA表达水平均低于空白对照组及慢病毒对照组（P?<0.05）；慢病毒转染组血浆TM、PAI-1蛋白表达水平均低于空白对照组及慢病毒对照组（P?<0.05）。结论 节律基因clif单核苷酸多态性位点rs1071592与老年AMI患者存在相关性。节律基因clif表达可降低慢病毒干扰小鼠的PAI-1及TM表达水平，增加血栓形成风险。

Abstract:

Objective To investigate the relationship between the single nucleotide polymorphism of the rhythm gene clif and acute myocardial infarction (AMI) in the elderly. Methods Retrospective analysis of 126 elderly patients with AMI who met the inclusion criteria from July 2016 to March 2018 in our hospital. 132 healthy subjects were selected as the control group. The SNaPshot was applied to detect the genotypic and allelic frequency of clif gene (rs2583913 and rs1071592) in the experimental group and control group. Lentivirus interference was used to silence the expression level of clif in mice. The expression levels of plasma thrombomodulin (TM) and plasminogen activator inhibitor-1 (PAI-1) mRNA in silent clif mice were observed. Results There was no statistical significance in distribution of genotypic and allelic frequency of rs2583913 in clif gene between experimental group and control group (P?>?0.05). There was statistical significance in distribution of genotypic and allelic frequency of rs1071592 in clif gene between experimental group andcontrol group (P?