放射治疗对肺癌患者NKG2D、HMGB1及DC细胞活性的影响*

Effect of radiation therapy on NKG2D, HMGB1 and DCs cell of lung cancer patients*

摘要:

目的 分析接受放射治疗（以下简称放疗）肺癌患者在放疗不同阶段外周血细胞因子NKG2D、高迁移率蛋白1（HMGB1）的变化及与树突状细胞（DC细胞）活性的相关性。方法 选取承德医学院附属医院30例肺癌放疗患者，分别在放疗前及放疗后不同时段通过ELISA检测NKG2D、HMGB1，通过免疫磁珠法检测DC细胞。结果 HMGB1、NKG2D的浓度于放疗2?000、4?000及6?000?cGy时逐渐下降（P?<0.05）。DC细胞活性差异无统计学意义（P?<0.05）。DC细胞与HMGB1、NKG2D无相关性（r?=-0.204和0.105，P?= 0.281和0.580）。结论 放射治疗可降低肺癌患者HMGB1、NKG2D的水平，激活免疫功能，但对肺癌患者外周血中DC细胞的活性无影响，外周血中DC细胞活性与HMGB1、NKG2D无相关。

Abstract:

Objective To analyze the dynamic changes of NKG2D, HMGB1 in peripherd blood of patients with lung cancer before and after different dose radiotherapy, and to analyze the relationship between DCs and NKG2D, HMGB1. Method A total of 30 patients with lung cancer were included in the study. The NKG2D and HMGB1 were detected by ELISA method, and the DCs were detected by MACS (magnetic activated cell sorting) before and after radiotherapy. Results HMGB1 and NKG2D gradually decreased after 2?000?cGy, 4?000?cGy and 6?000?cGy radiotherapy (P??0.05). There was no correlation between DCs and HMGB1, NKG2D (r?=?-0.204 and 0.105, P?=?0.281 and 0.580). Conclusion Radiation therapy can reduce the level of HMGB1 and NKG2D, and activate the cellular immune function. However, radiotherapy has no effect on the activity of DCs in peripheral blood of patients with lung cancer, and there was no correlation between DCs and cytokines (HMGB1, NKG2D) in peripheral blood.