纳尔逊海湾病毒μNS 蛋白 与σNS 蛋白的表达分析

Expression and analysis of Nelson Bay orthoreovirus μNS protein and σNS protein

摘要:

目的 研究呼肠孤病毒科纳尔逊海湾病毒非结构蛋白μNS 与σNS 在感染及转染细胞中的表 达和分析。方法 在感染细胞中，通过免疫荧光方法在共聚焦显微镜下观察μNS 蛋白的表达，以及μNS 和σNS 共定位情况；同时通过转染质粒的方法观察非感染细胞中μNS 和σNS 的表达及共定位情况。 结果 μNS 蛋白在感染细胞和单独转染质粒细胞中都形成包涵体样结构，σNS 和μNS 蛋白相互作用， σNS 和μNS 蛋白共定位存在于包涵体样结构内。结论 μNS 不仅可以在病毒感染细胞中，而且可以在单 独转染的细胞中形成包涵体样结构，σNS 可以与μNS 相互作用，共定位于感染和转染细胞的包涵体样结 构中。

Abstract:

Objective To study the expression and analysis of Nelson Bay orthoreovirus non-structural protein μNS and σNS in infected and transfected cells. Methods The expression of μNS protein and the colocalization of μNS with another non-structural protein σNS were observed by immunofluorescence assay through confocal microscopy in infected cells. The expression and colocalization of μNS and σNS were observed in non-infected cells which were transfected by plasmids. Results μNS protein formed inclusion bodies in both infected and transfected cells. The σNS protein interacted with μNS protein. σNS and μNS protein were colocalized in inclusion bodies. Conclusion μNS can form inclusion bodies not only in virus-infected cells but also in cells transfected. σNS can interact and was colocalize with μNS in the inclusion bodies of infected and transfected cells.