小窝蛋白-1在白藜芦醇治疗小鼠急性肺损伤中的作用研究*

Resveratrol participates in the protective effect of acute lung injury in mice through caveolin-1*

摘要:

目的 研究小窝蛋白-1（Cav-1）在急性肺损伤（ALI）中的作用以及白藜芦醇抗炎的可能分子机制。方法 将50只BALB/c小鼠随机分为正常组、ALI组、ALI+白藜芦醇干预组、Cav-1过表达的ALI组、Cav-1过表达的ALI+白藜芦醇干预组，每组10只。采用脂多糖（LPS）气管滴注复制小鼠ALI模型，尾静脉注射GV287-Cav-1过表达载体复制Cav-1过表达小鼠，白藜芦醇腹腔给药干预小鼠ALI模型。模型复制成功后取肺组织观察病理组织学变化，采用免疫组织化学和Western blotting检测Cav-1在肺组织中的表达，提取小鼠血清检测肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）。结果 过表达Cav-1能促进ALI小鼠释放更多的TNF-α、IL-6（P?<0.05），白藜芦醇能降低Cav-1蛋白的表达水平，改善ALI，抑制TNF-α、IL-6的释放（P?<0.05），减轻肺组织炎症损伤。结论 白藜芦醇可能通过抑制Cav-1的表达起到对小鼠ALI的治疗作用。

Abstract:

Objective To investigate the role of caveolin-1 (Cav-1) in acute lung injury and the anti-inflammatory effect of resveratrol in molecular mechanism. Methods Fifty BALB/c mice were randomly divided into five groups, the normal group (n?=?10), ALI group (n?=?10), resveratrol interference group (n?=?10), ALI group with over-expression of Cav-1 (n?=?10), and ALI with over-expression of Cav-1+resveratrol interference group (n?=?10). ALI mice model was treated with tracheal instillation by lipopolysaccharides (LPS). Overexpression of Cav-1 was established by intravenous injection of GV287-Cav-1 over expression vector through tail vein, and ALI mice models were intervened by intraperitoneal administration of resveratrol. After the model was established, the lung tissue was taken for pathological examination, then immunohistochemistry and Western blotting were taken to detected the expression of Cav-1 in lung tissue, and the mouse serum were taken to test the inflammatory factors TNF-α and IL-6. Results Overexpression of Cav-1 could promote the release of TNF-α and IL-6 in ALI mice (P?