心脏不停跳大鼠单肺体外循环肺损伤模型的建立

Establishment of a single-lung cardiopulmonary-bypass lung injury model in rats with beating heart

摘要:

目的 建立心脏不停跳大鼠单肺体外循环（CPB）肺损伤模型，为研究CPB肺损伤的发病机制及保护措施提供实验平台。方法 健康成年SD大鼠24只，随机分为单纯开胸组（T组）、单纯CPB组（C组）和缺血再灌注组（IR组），每组8只。T组仅开胸，C组开胸后建立CPB，IR组建立CPB期间行左肺缺血再灌注损伤。在CPB前（T1）、开放左肺门即刻（T2）及实验结束时（T3）行动脉血气分析，记录红细胞压积（Hct）、血乳酸（Lac）和肺功能的变化。实验结束时，检测血清肿瘤坏死因子α（TNF-α）和白细胞介素-1β（IL-1β）的含量；剪取左肺组织，观察左肺组织的病理变化。结果 CPB后Hct下降（P?<0.05）。与T组比较，实验结束时C组和IR组肺功能下降，Lac和肺损伤评分较T组高（P?<0.05）；上述变化在IR组中最为突出。IR组中血液和肺组织的IL-1β、TNF-α含量最多（P?<0.05）。结论 该自创实验动物模型建立成功，能模拟临床CPB肺损伤的病理生理变化，对CPB肺保护相关研究有着积极的推动作用。

Abstract:

Objective To establish a single-lung cardiopulmonary-bypass (CPB) lung injury model in rats with beating heart, and to provide an ideal experimental platform for studying the mechanism and protective measures of CPB lung injury. Methods Twenty-four rats were divided into 3 groups (n?=?8, each): the thoracotomy group (T group), the CPB group (C group) and the left lung ischemia/reperfusion injury group (IR group). T Group received sternotomy only; CPB was exerted in C group; in IR group, the left lung ischemia/reperfusion injury was conducted during CPB. Arterial blood gases were analyzed before CPB (T1), at the onset of opening the left hilus pulmonis (T2) and 90 min after CPB (T3) to observe the changes of hematocrit, lactate and lung function. At T3, serum TNF-α and IL-1β were measured, and the expressions of TNF-α and IL-1β and pathological change of the left lung tissue were observed. Results Hematocrit was decreased significantly after CPB (P?